Abstract
Objectives: Biofilm-forming Staphylococcus epidermidis is a prevalent cause of peritonitis during peritoneal dialysis. We compared the efficacy of a synthetic antimicrobial peptidomimetic (Ltx21) versus vancomycin in a murine model mimicking a device-related peritonitis. Methods: Silicone implants, pre-colonized with an S. epidermidis biofilm, were inserted into the peritoneal cavity of BALB/c mice. Three groups (36 mice in each) with pre-colonized implants received intraperitoneal treatment with Ltx21, vancomycin or placebo. Micewereeuthanizedonday3(n1/412),day6(n1/412) orday8(n1/412) post-implantation. Controlswere mice with sterile implants (n1/418) and mice without surgery (n1/46). Bacterial reductions in cfu were analysed from implants and peritoneal fluid (PF). Inflammatory responses in serum and PFwere measured. Results: Vancomycin resulted in a stronger reduction in cfu counts, both on pre-colonized implants and in PF, compared with Ltx21 and placebo. Complete bacterial clearance of the implantswas not achieved in anyof the groups. The implants pre-colonized with S. epidermidis 1457 resulted in a low-grade peritonitis. We observed, onlyon day 6, a significant increase in the PF leucocyte count in the group with pre-colonized implants compared with the group with sterile implants (P1/40.0364). Conclusions: Treatment with vancomycin or Ltx21 was not sufficient to achieve complete bacterial clearance of implants, underlining the difficulties of treating such infections. The low-grade infectionmayattenuate the inflammatory response and contribute to impaired bacterial clearance
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | The Journal of antimicrobial chemotherapy |
| Vol/bind | 68 |
| Udgave nummer | 9 |
| Sider (fra-til) | 2106-10 |
| Antal sider | 5 |
| ISSN | 0305-7453 |
| DOI | |
| Status | Udgivet - sep. 2013 |