Effects of phthalates on the human corneal endothelial cell line B4G12

Tanja Krüger; Yi Cao; Søren K. Kjærgaard; Lisbeth E Knudsen; Eva Cecilie Bonefeld-Jørgensen

doi:10.1177/1091581812449660

Effects of phthalates on the human corneal endothelial cell line B4G12

Tanja Krüger, Yi Cao, Søren K. Kjærgaard, Lisbeth E Knudsen, Eva Cecilie Bonefeld-Jørgensen

13 Citationer (Scopus)

Abstract

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

Originalsprog	Engelsk
Tidsskrift	International Journal of Toxicology
Vol/bind	31
Udgave nummer	4
Sider (fra-til)	364-71
Antal sider	8
ISSN	1091-5818
DOI	https://doi.org/10.1177/1091581812449660
Status	Udgivet - jul. 2012

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10.1177/1091581812449660

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title = "Effects of phthalates on the human corneal endothelial cell line B4G12",

abstract = "Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.",

author = "Tanja Kr{\"u}ger and Yi Cao and Kj{\ae}rgaard, {S{\o}ren K.} and Knudsen, {Lisbeth E} and Bonefeld-J{\o}rgensen, {Eva Cecilie}",

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T1 - Effects of phthalates on the human corneal endothelial cell line B4G12

AU - Krüger, Tanja

AU - Cao, Yi

AU - Kjærgaard, Søren K.

AU - Knudsen, Lisbeth E

AU - Bonefeld-Jørgensen, Eva Cecilie

PY - 2012/7

Y1 - 2012/7

N2 - Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

AB - Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

U2 - 10.1177/1091581812449660

DO - 10.1177/1091581812449660

M3 - Journal article

C2 - 22723514

SN - 1091-5818

VL - 31

SP - 364

EP - 371

JO - International Journal of Toxicology

JF - International Journal of Toxicology

IS - 4

ER -

Effects of phthalates on the human corneal endothelial cell line B4G12

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