Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients: results from a randomized, placebo-controlled trial

TY - JOUR

T1 - Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients

T2 - results from a randomized, placebo-controlled trial

AU - Aladdin, H

AU - Ullum, H

AU - Schjerling, P

AU - Skov Jensen, M

AU - Dam Nielsen, S.

AU - Mathiesen, L

AU - Gerstoft, J

AU - Skinhøj, P

AU - Klarlund Pedersen, B

PY - 2000/8

Y1 - 2000/8

N2 - Telomeres are unique terminal chromosomal structures, the length of which has been shown to decrease with cell division in vitro and with increased age in vivo for human somatic cells. In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is primarily found in CD8+ T cells, and not in CD4+ T cells. In this double-blind placebo-controlled study, we investigated the effect of granulocyte colony stimulating factor (G-CSF) treatment combined with highly active antiretroviral therapy (HAART) on mean telomere length in peripheral blood mononuclear cells (PBMC). The terminal restriction fragment (TRF) length showed no changes during G-CSF treatment although the number of lymphocytes increased significantly. The mean TRF length correlated positively (R = 0.552, P = 0.009) and negatively (R = -0.503, P = 0.02) to the proportion of CD4+ memory and naïve cells, respectively. Our data suggest that during G-CSF treatment lymphocytes are recruited by a combination of central and peripheral proliferation.

AB - Telomeres are unique terminal chromosomal structures, the length of which has been shown to decrease with cell division in vitro and with increased age in vivo for human somatic cells. In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is primarily found in CD8+ T cells, and not in CD4+ T cells. In this double-blind placebo-controlled study, we investigated the effect of granulocyte colony stimulating factor (G-CSF) treatment combined with highly active antiretroviral therapy (HAART) on mean telomere length in peripheral blood mononuclear cells (PBMC). The terminal restriction fragment (TRF) length showed no changes during G-CSF treatment although the number of lymphocytes increased significantly. The mean TRF length correlated positively (R = 0.552, P = 0.009) and negatively (R = -0.503, P = 0.02) to the proportion of CD4+ memory and naïve cells, respectively. Our data suggest that during G-CSF treatment lymphocytes are recruited by a combination of central and peripheral proliferation.

KW - Adult

KW - Aged

KW - Anti-HIV Agents

KW - CD4 Lymphocyte Count

KW - Double-Blind Method

KW - Drug Therapy, Combination

KW - Female

KW - Granulocyte Colony-Stimulating Factor

KW - HIV Infections

KW - Humans

KW - In Vitro Techniques

KW - Leukocytes, Mononuclear

KW - Lymphocytes

KW - Male

KW - Middle Aged

KW - Monocytes

KW - Telomere

KW - Clinical Trial

KW - Journal Article

KW - Randomized Controlled Trial

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 10931390

SN - 0301-6323

VL - 52

SP - 212

EP - 216

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 2

ER -