TY - JOUR
T1 - Effects of biochar amendments on antibiotic resistome of the soil and collembolan gut
AU - Ding, Jing
AU - Yin, Yue
AU - Sun, An Qi
AU - Lassen, Simon Bo
AU - Li, Gang
AU - Zhu, Dong
AU - Ke, Xin
PY - 2019/9/5
Y1 - 2019/9/5
N2 - A diverse array of ARGs has been detected in the guts of soil fauna residing in farmland soil. Biochar has been widely used in farmland for soil remediation and improvement of soil quality; however, the effects of biochar amendment on the gut-associated ARGs of soil fauna remain unclear. In the present study, collembolans were cultivated in soils amended with 6 types of biochars. High-throughput qPCR was used to establish ARG profiles of the collembolan guts as well as the surrounding soils. A total of 73 and 162 subtypes of ARGs were detected in the collembolan guts and soils, respectively. Biochar amendment significantly altered the ARG compositions of the collembolan guts and soils, in a biochar quality-dependent manner. However, only manure-derived biochar, which contained elevated concentrations of heavy metals, increased the relative abundance of gut-associated ARGs. Changes in the gut microbial community, MGEs and biochar properties explained 84% of the total ARG variations in the collembolan guts. The findings of this study suggested that biochar properties should receive more attention, as high doses of heavy metals in biochar could increase the abundance of ARGs in collembolan guts, thereby contributing to the spread of ARGs in the environment through collembolan movement.
AB - A diverse array of ARGs has been detected in the guts of soil fauna residing in farmland soil. Biochar has been widely used in farmland for soil remediation and improvement of soil quality; however, the effects of biochar amendment on the gut-associated ARGs of soil fauna remain unclear. In the present study, collembolans were cultivated in soils amended with 6 types of biochars. High-throughput qPCR was used to establish ARG profiles of the collembolan guts as well as the surrounding soils. A total of 73 and 162 subtypes of ARGs were detected in the collembolan guts and soils, respectively. Biochar amendment significantly altered the ARG compositions of the collembolan guts and soils, in a biochar quality-dependent manner. However, only manure-derived biochar, which contained elevated concentrations of heavy metals, increased the relative abundance of gut-associated ARGs. Changes in the gut microbial community, MGEs and biochar properties explained 84% of the total ARG variations in the collembolan guts. The findings of this study suggested that biochar properties should receive more attention, as high doses of heavy metals in biochar could increase the abundance of ARGs in collembolan guts, thereby contributing to the spread of ARGs in the environment through collembolan movement.
KW - Biochar
KW - Gut microbiota
KW - Resistome
KW - Soil fauna
UR - http://www.scopus.com/inward/record.url?scp=85066289861&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2019.05.089
DO - 10.1016/j.jhazmat.2019.05.089
M3 - Journal article
C2 - 31163347
AN - SCOPUS:85066289861
SN - 0304-3894
VL - 377
SP - 186
EP - 194
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -