Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study

Lena Leder; Marjukka Kolehmainen; Ingunn Narverud; Ingrid Dahlman; Mari C.W. Myhrstad; Vanessa de Mello; Jussi Paananen; Carsten Carlberg; Ursula S Schwab; Karl-Heinz Herzig; Lieselotte Cloetens; Matilda Ulmius Storm; Janne Hukkanen; Markku J Savolainen; Fredrik Rosqvist; Kjeld Hermansen; Lars Ove Dragsted; Ingibjorg Gunnarsdottir; Inga Thorsdottir; Ulf Risérus; Björn Åkesson; Magne Thoresen; Peter Arner; Kaisa S Poutanen; Matti Uusitupa; Kirsten B Holven; Stine M Ulven

doi:10.1186/s12263-016-0521-4

Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study

Lena Leder, Marjukka Kolehmainen, Ingunn Narverud, Ingrid Dahlman, Mari C.W. Myhrstad, Vanessa de Mello, Jussi Paananen, Carsten Carlberg, Ursula S Schwab, Karl-Heinz Herzig, Lieselotte Cloetens, Matilda Ulmius Storm, Janne Hukkanen, Markku J Savolainen, Fredrik Rosqvist, Kjeld Hermansen, Lars Ove Dragsted, Ingibjorg Gunnarsdottir, Inga Thorsdottir, Ulf RisérusBjörn Åkesson, Magne Thoresen, Peter Arner, Kaisa S Poutanen, Matti Uusitupa, Kirsten B Holven, Stine M Ulven

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Abstract

Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n =26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L^-1, and baseline body mass index <39 kg m^-2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

Originalsprog	Engelsk
Artikelnummer	3
Tidsskrift	Genes & Nutrition
Vol/bind	11
Antal sider	13
ISSN	1555-8932
DOI	https://doi.org/10.1186/s12263-016-0521-4
Status	Udgivet - 2016

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10.1186/s12263-016-0521-4Licens: CC BY

Leder et al_ Genes & Nutrition_2016_Vol 11_Article 3Forlagets udgivne version, 983 KBLicens: CC BY

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959556/pdf/12263_2016_Article_521.pdfLicens: CC BY

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Leder, L., Kolehmainen, M., Narverud, I., Dahlman, I., Myhrstad, M. C. W., de Mello, V., Paananen, J., Carlberg, C., Schwab, U. S., Herzig, K-H., Cloetens, L., Storm, M. U., Hukkanen, J., Savolainen, M. J., Rosqvist, F., Hermansen, K., Dragsted, L. O., Gunnarsdottir, I., Thorsdottir, I., ... Ulven, S. M. (2016). Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study. Genes & Nutrition, 11, [3]. https://doi.org/10.1186/s12263-016-0521-4

Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome : a SYSDIET sub-study. / Leder, Lena; Kolehmainen, Marjukka; Narverud, Ingunn et al.

I: Genes & Nutrition, Bind 11, 3, 2016.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Leder, L, Kolehmainen, M, Narverud, I, Dahlman, I, Myhrstad, MCW, de Mello, V, Paananen, J, Carlberg, C, Schwab, US, Herzig, K-H, Cloetens, L, Storm, MU, Hukkanen, J, Savolainen, MJ, Rosqvist, F, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Thorsdottir, I, Risérus, U, Åkesson, B, Thoresen, M, Arner, P, Poutanen, KS, Uusitupa, M, Holven, KB & Ulven, SM 2016, 'Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study', Genes & Nutrition, bind 11, 3. https://doi.org/10.1186/s12263-016-0521-4

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title = "Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study",

abstract = "Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n =26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L-1, and baseline body mass index <39 kg m-2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.",

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author = "Lena Leder and Marjukka Kolehmainen and Ingunn Narverud and Ingrid Dahlman and Myhrstad, {Mari C.W.} and {de Mello}, Vanessa and Jussi Paananen and Carsten Carlberg and Schwab, {Ursula S} and Karl-Heinz Herzig and Lieselotte Cloetens and Storm, {Matilda Ulmius} and Janne Hukkanen and Savolainen, {Markku J} and Fredrik Rosqvist and Kjeld Hermansen and Dragsted, {Lars Ove} and Ingibjorg Gunnarsdottir and Inga Thorsdottir and Ulf Ris{\'e}rus and Bj{\"o}rn {\AA}kesson and Magne Thoresen and Peter Arner and Poutanen, {Kaisa S} and Matti Uusitupa and Holven, {Kirsten B} and Ulven, {Stine M}",

note = "CURIS 2016 NEXS 207",

year = "2016",

doi = "10.1186/s12263-016-0521-4",

language = "English",

volume = "11",

journal = "Genes & Nutrition",

issn = "1555-8932",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome

T2 - a SYSDIET sub-study

AU - Leder, Lena

AU - Kolehmainen, Marjukka

AU - Narverud, Ingunn

AU - Dahlman, Ingrid

AU - Myhrstad, Mari C.W.

AU - de Mello, Vanessa

AU - Paananen, Jussi

AU - Carlberg, Carsten

AU - Schwab, Ursula S

AU - Herzig, Karl-Heinz

AU - Cloetens, Lieselotte

AU - Storm, Matilda Ulmius

AU - Hukkanen, Janne

AU - Savolainen, Markku J

AU - Rosqvist, Fredrik

AU - Hermansen, Kjeld

AU - Dragsted, Lars Ove

AU - Gunnarsdottir, Ingibjorg

AU - Thorsdottir, Inga

AU - Risérus, Ulf

AU - Åkesson, Björn

AU - Thoresen, Magne

AU - Arner, Peter

AU - Poutanen, Kaisa S

AU - Uusitupa, Matti

AU - Holven, Kirsten B

AU - Ulven, Stine M

N1 - CURIS 2016 NEXS 207

PY - 2016

Y1 - 2016

N2 - Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n =26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L-1, and baseline body mass index <39 kg m-2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

AB - Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n =26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L-1, and baseline body mass index <39 kg m-2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

KW - Journal Article

U2 - 10.1186/s12263-016-0521-4

DO - 10.1186/s12263-016-0521-4

M3 - Journal article

C2 - 27482295

SN - 1555-8932

VL - 11

JO - Genes & Nutrition

JF - Genes & Nutrition

M1 - 3

ER -

Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study

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