Effect of plant extracts and an essential oil on the control of brown spot disease, tillering, number of panicles and yield increase in rice

TY - JOUR

T1 - Effect of plant extracts and an essential oil on the control of brown spot disease, tillering, number of panicles and yield increase in rice

AU - Nguefack, Julienne

AU - Wulff, Ednar Gadelha

AU - Dongmo, J. Blaise Lekagne

AU - Fouelefack, F. Romain

AU - Fotio, Daniel

AU - Mbo, Joseph

AU - Torp, Jan

PY - 2013/12/1

Y1 - 2013/12/1

N2 - The effects of essential oils (EO), cold water (CWE), hot water (HWE) and ethanol (ETHE) extracts of Callistemon citrinus L. and Cymbopogon citratus (DC) Stapf on the radial growth of Alternaria padwickii (Ganguly) M.B. Ellis and Bipolaris oryzae (Breda de Haan) Shoemaker, the control of brown spot disease, the tillering, the number of panicles and the yield increase in rice were evaluated under laboratory and field conditions. In vitro, the growth of both fungi was completely inhibited by the EO of C. citrinus and C. citratus at 4,520 μg/ml and 452 μg/ml, respectively. For solvent extracts, the ETHE of C. citrinus was the most active and inhibited 80-85 % of the fungal growth followed by the CWE of C. citratus with 77 % and 36 % diameter reduction against B. oryzae and A. padwickii, respectively at 10 000 μg/ml. Under laboratory conditions, seed treatment with the EO of C. citrinus reduced the incidence of B. oryzae in seeds by 85-100 % compared to the non-treated controls. Similarly, the seed treatment increased the germination of an irrigated rice cultivar by 10.6 %, whereas the percentage of germinated seeds of upland rice was not significantly affected. The highest germination (85-94 %) was found in the non-treated and treated samples with a low incidence (0-4 %) of B. oryzae. Under field conditions, the combined use of the essential oil of C. citrinus as a seed treatment and spraying the plants with 2 % ethanol followed by 2 % (w/v) aqueous extracts of C. citrinus or C. citratus increased the emergence, tillering, panicles/plant and the grain yield by 25-55 % of the irrigated rice. In addition, the brown spot severity was reduced by 36-42 %. For the upland rice, the treatments led to similar results with the grain yield increase of 54-137 % and 20-80 % reduction in the brown spot severity. From our results, we concluded that the EO and solvent extracts of C. citrinus and C. citratus have potential as control agents against brown spot and other seed-borne fungal diseases in rice under both conventional and organic farming.

AB - The effects of essential oils (EO), cold water (CWE), hot water (HWE) and ethanol (ETHE) extracts of Callistemon citrinus L. and Cymbopogon citratus (DC) Stapf on the radial growth of Alternaria padwickii (Ganguly) M.B. Ellis and Bipolaris oryzae (Breda de Haan) Shoemaker, the control of brown spot disease, the tillering, the number of panicles and the yield increase in rice were evaluated under laboratory and field conditions. In vitro, the growth of both fungi was completely inhibited by the EO of C. citrinus and C. citratus at 4,520 μg/ml and 452 μg/ml, respectively. For solvent extracts, the ETHE of C. citrinus was the most active and inhibited 80-85 % of the fungal growth followed by the CWE of C. citratus with 77 % and 36 % diameter reduction against B. oryzae and A. padwickii, respectively at 10 000 μg/ml. Under laboratory conditions, seed treatment with the EO of C. citrinus reduced the incidence of B. oryzae in seeds by 85-100 % compared to the non-treated controls. Similarly, the seed treatment increased the germination of an irrigated rice cultivar by 10.6 %, whereas the percentage of germinated seeds of upland rice was not significantly affected. The highest germination (85-94 %) was found in the non-treated and treated samples with a low incidence (0-4 %) of B. oryzae. Under field conditions, the combined use of the essential oil of C. citrinus as a seed treatment and spraying the plants with 2 % ethanol followed by 2 % (w/v) aqueous extracts of C. citrinus or C. citratus increased the emergence, tillering, panicles/plant and the grain yield by 25-55 % of the irrigated rice. In addition, the brown spot severity was reduced by 36-42 %. For the upland rice, the treatments led to similar results with the grain yield increase of 54-137 % and 20-80 % reduction in the brown spot severity. From our results, we concluded that the EO and solvent extracts of C. citrinus and C. citratus have potential as control agents against brown spot and other seed-borne fungal diseases in rice under both conventional and organic farming.

KW - Cochliobolus miyabeanus

KW - Disease management

KW - Essential oils

KW - Helminthosporiosis

KW - Plant extracts

KW - Rice

U2 - 10.1007/s10658-013-0298-0

DO - 10.1007/s10658-013-0298-0

M3 - Journal article

SN - 0929-1873

VL - 137

SP - 871

EP - 882

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

IS - 4

ER -