TY - JOUR
T1 - Effect of lifelong resveratrol supplementation and exercise training on skeletal muscle oxidative capacity in aging mice
T2 - impact of PGC-1α
AU - Ringholm, Stine
AU - Olesen, Jesper
AU - Pedersen, Jesper Thorhauge
AU - Brandt, Christina Tingbjerg
AU - Halling, Jens Frey
AU - Hellsten, Ylva
AU - Prats Gavalda, Clara
AU - Pilegaard, Henriette
N1 - CURIS 2013 NEXS 225
PY - 2013/11
Y1 - 2013/11
N2 - Background: The present study tested the hypothesis that lifelong resveratrol (RSV) supplementation counteracts an age-associated decrease in skeletal muscle oxidative capacity through peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α and that RSV combined with lifelong exercise training (EX) exerts additive effects through PGC-1α in mice. Methods: 3. month old PGC-1α whole body knockout (KO) and wild type (WT) littermate mice were placed in cages with or without running wheel and fed either standard chow or standard chow with RSV supplementation (4. g/kg food) for 12. months. Young (3. months of age), sedentary mice on standard chow served as young controls. A graded running performance test and a glucose tolerance test were performed 2 and 1. week, respectively, before euthanization where quadriceps and extensor digitorum longus (EDL) muscles were removed. Results: In PGC-1α KO mice, quadriceps citrate synthase (CS) activity, mitochondrial (mt)DNA content as well as pyruvate dehydrogenase (PDH)-E1α, cytochrome (Cyt) c and vascular endothelial growth factor (VEGF) protein content were 20-75% lower and, EDL capillary-to-fiber (C:F) ratio was 15-30% lower than in WT mice. RSV and/or EX had no effect on the C:F ratio in EDL. CS activity (P. = 0.063) and mtDNA content (P. = 0.013) decreased with age in WT mice, and CS activity, mtDNA content, PDH-E1α protein and VEGF protein increased ~. 1.5-1.8-fold with lifelong EX in WT, but not in PGC-1α KO mice, while RSV alone had no significant effect on these proteins. Conclusion: Lifelong EX increased activity/content of oxidative proteins, mtDNA and angiogenic proteins in skeletal muscle through PGC-1α, while RSV supplementation alone had no effect. Combining lifelong EX and RSV supplementation had no additional effect on skeletal muscle oxidative and angiogenic proteins.
AB - Background: The present study tested the hypothesis that lifelong resveratrol (RSV) supplementation counteracts an age-associated decrease in skeletal muscle oxidative capacity through peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α and that RSV combined with lifelong exercise training (EX) exerts additive effects through PGC-1α in mice. Methods: 3. month old PGC-1α whole body knockout (KO) and wild type (WT) littermate mice were placed in cages with or without running wheel and fed either standard chow or standard chow with RSV supplementation (4. g/kg food) for 12. months. Young (3. months of age), sedentary mice on standard chow served as young controls. A graded running performance test and a glucose tolerance test were performed 2 and 1. week, respectively, before euthanization where quadriceps and extensor digitorum longus (EDL) muscles were removed. Results: In PGC-1α KO mice, quadriceps citrate synthase (CS) activity, mitochondrial (mt)DNA content as well as pyruvate dehydrogenase (PDH)-E1α, cytochrome (Cyt) c and vascular endothelial growth factor (VEGF) protein content were 20-75% lower and, EDL capillary-to-fiber (C:F) ratio was 15-30% lower than in WT mice. RSV and/or EX had no effect on the C:F ratio in EDL. CS activity (P. = 0.063) and mtDNA content (P. = 0.013) decreased with age in WT mice, and CS activity, mtDNA content, PDH-E1α protein and VEGF protein increased ~. 1.5-1.8-fold with lifelong EX in WT, but not in PGC-1α KO mice, while RSV alone had no significant effect on these proteins. Conclusion: Lifelong EX increased activity/content of oxidative proteins, mtDNA and angiogenic proteins in skeletal muscle through PGC-1α, while RSV supplementation alone had no effect. Combining lifelong EX and RSV supplementation had no additional effect on skeletal muscle oxidative and angiogenic proteins.
U2 - 10.1016/j.exger.2013.08.012
DO - 10.1016/j.exger.2013.08.012
M3 - Journal article
C2 - 23994519
SN - 0531-5565
VL - 48
SP - 1311
EP - 1318
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 11
ER -