Early development of the porcine embryo: the importance of cell signalling in development of pluripotent cell lines

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    Abstract

    Understanding the cell signalling events that govern cell renewal in porcine pluripotent cells may help improve culture conditions and allow for establishment of bona fide porcine embryonic stem cells (pESC) and stable porcine induced pluripotent stem cells (piPSC). This review investigates cell signalling in the porcine preimplantation embryo containing either the inner cell mass or epiblast, with particular emphasis on fibroblast growth factor, SMAD, WNT and Janus tyrosine kinases/signal transducers and activators of transcription signalling. It is clear that key differences exist in the cell signalling events that govern pluripotency in this species compared with similar embryonic stages in mouse and human. The fact that bona fide pESC have still not been produced and that piPSC cannot survive in culture following the silencing or downregulation of the reprogramming transgenes suggest that culture conditions are not optimal. Unravelling the factor/s that regulate pluripotency in porcine embryos will pave the way for future establishment of stable pluripotent stem cell lines.

    OriginalsprogEngelsk
    TidsskriftReproduction, Fertility and Development
    Vol/bind25
    Udgave nummer1
    Sider (fra-til)94-102
    Antal sider9
    ISSN1031-3613
    DOI
    StatusUdgivet - 2013

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