Early aberrations in chromatin dynamics in embryos produced under In vitro conditions

Rahul Shahaji Deshmukh; Olga Østrup; Frantisek Strejcek; Morten Vejlsted; Andrea Lucas-Hahn; Bjorn Petersen; Juan Li; Henrik Callesen; Heiner Niemann; Poul Hyttel

doi:10.1089/cell.2011.0069

Early aberrations in chromatin dynamics in embryos produced under In vitro conditions

Rahul Shahaji Deshmukh, Olga Østrup, Frantisek Strejcek, Morten Vejlsted, Andrea Lucas-Hahn, Bjorn Petersen, Juan Li, Henrik Callesen, Heiner Niemann, Poul Hyttel

13 Citationer (Scopus)

Abstract

In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos developed in vivo (IV) and compared this physiological standard to that of embryos produced by IVF, parthenogenetic activation (PA), or SCNT. In contrast to IV embryos, chromatin spatial and temporal dynamics in PA, IVF, and SCNT embryos were altered; starting with aberrant chromatin-nuclear envelope interactions at the two-cell stage, delayed chromatin decondensation and nucleolar development at the four-cell stage, and ultimately culminating in failure of proper first lineage segregation at the blastocyst stage, demonstrated by poorly defined inner cell mass. Interestingly, in vitro produced (IVP) embryos also lacked a heterochromatin halo around nucleolar precursors, indicating imperfections in global chromatin remodeling after fertilization/activation. Porcine IV-produced zygotes and embryos display a well-synchronized pattern of chromatin dynamics compatible with genome activation and regular nucleolar formation at the four-cell stage. Production of porcine embryos under in vitro conditions by IVF, PA, or SCNT is associated with altered chromatin remodeling, delayed nucleolar formation, and poorly defined lineage segregation at the blastocyst stage, which in turn may impair their developmental capacity.

Originalsprog	Engelsk
Tidsskrift	Cellular Reprogramming
Vol/bind	14
Udgave nummer	3
Sider (fra-til)	225-234
Antal sider	10
ISSN	2152-4971
DOI	https://doi.org/10.1089/cell.2011.0069
Status	Udgivet - 1 jun. 2012

Adgang til dokumentet

10.1089/cell.2011.0069

http://online.liebertpub.com/doi/pdfplus/10.1089/cell.2011.0069

Citationsformater

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title = "Early aberrations in chromatin dynamics in embryos produced under In vitro conditions",

abstract = "In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos developed in vivo (IV) and compared this physiological standard to that of embryos produced by IVF, parthenogenetic activation (PA), or SCNT. In contrast to IV embryos, chromatin spatial and temporal dynamics in PA, IVF, and SCNT embryos were altered; starting with aberrant chromatin-nuclear envelope interactions at the two-cell stage, delayed chromatin decondensation and nucleolar development at the four-cell stage, and ultimately culminating in failure of proper first lineage segregation at the blastocyst stage, demonstrated by poorly defined inner cell mass. Interestingly, in vitro produced (IVP) embryos also lacked a heterochromatin halo around nucleolar precursors, indicating imperfections in global chromatin remodeling after fertilization/activation. Porcine IV-produced zygotes and embryos display a well-synchronized pattern of chromatin dynamics compatible with genome activation and regular nucleolar formation at the four-cell stage. Production of porcine embryos under in vitro conditions by IVF, PA, or SCNT is associated with altered chromatin remodeling, delayed nucleolar formation, and poorly defined lineage segregation at the blastocyst stage, which in turn may impair their developmental capacity.",

author = "Deshmukh, {Rahul Shahaji} and Olga {\O}strup and Frantisek Strejcek and Morten Vejlsted and Andrea Lucas-Hahn and Bjorn Petersen and Juan Li and Henrik Callesen and Heiner Niemann and Poul Hyttel",

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T1 - Early aberrations in chromatin dynamics in embryos produced under In vitro conditions

AU - Deshmukh, Rahul Shahaji

AU - Østrup, Olga

AU - Strejcek, Frantisek

AU - Vejlsted, Morten

AU - Lucas-Hahn, Andrea

AU - Petersen, Bjorn

AU - Li, Juan

AU - Callesen, Henrik

AU - Niemann, Heiner

AU - Hyttel, Poul

PY - 2012/6/1

Y1 - 2012/6/1

N2 - In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos developed in vivo (IV) and compared this physiological standard to that of embryos produced by IVF, parthenogenetic activation (PA), or SCNT. In contrast to IV embryos, chromatin spatial and temporal dynamics in PA, IVF, and SCNT embryos were altered; starting with aberrant chromatin-nuclear envelope interactions at the two-cell stage, delayed chromatin decondensation and nucleolar development at the four-cell stage, and ultimately culminating in failure of proper first lineage segregation at the blastocyst stage, demonstrated by poorly defined inner cell mass. Interestingly, in vitro produced (IVP) embryos also lacked a heterochromatin halo around nucleolar precursors, indicating imperfections in global chromatin remodeling after fertilization/activation. Porcine IV-produced zygotes and embryos display a well-synchronized pattern of chromatin dynamics compatible with genome activation and regular nucleolar formation at the four-cell stage. Production of porcine embryos under in vitro conditions by IVF, PA, or SCNT is associated with altered chromatin remodeling, delayed nucleolar formation, and poorly defined lineage segregation at the blastocyst stage, which in turn may impair their developmental capacity.

AB - In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos developed in vivo (IV) and compared this physiological standard to that of embryos produced by IVF, parthenogenetic activation (PA), or SCNT. In contrast to IV embryos, chromatin spatial and temporal dynamics in PA, IVF, and SCNT embryos were altered; starting with aberrant chromatin-nuclear envelope interactions at the two-cell stage, delayed chromatin decondensation and nucleolar development at the four-cell stage, and ultimately culminating in failure of proper first lineage segregation at the blastocyst stage, demonstrated by poorly defined inner cell mass. Interestingly, in vitro produced (IVP) embryos also lacked a heterochromatin halo around nucleolar precursors, indicating imperfections in global chromatin remodeling after fertilization/activation. Porcine IV-produced zygotes and embryos display a well-synchronized pattern of chromatin dynamics compatible with genome activation and regular nucleolar formation at the four-cell stage. Production of porcine embryos under in vitro conditions by IVF, PA, or SCNT is associated with altered chromatin remodeling, delayed nucleolar formation, and poorly defined lineage segregation at the blastocyst stage, which in turn may impair their developmental capacity.

U2 - 10.1089/cell.2011.0069

DO - 10.1089/cell.2011.0069

M3 - Journal article

C2 - 22468997

SN - 2152-4971

VL - 14

SP - 225

EP - 234

JO - Cellular Reprogramming

JF - Cellular Reprogramming

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