@article{2fc1cd10368311df8ed1000ea68e967b,
title = "Dynamic strength of the interaction between lung surfactant protein D (SP-D) and saccharide ligands",
abstract = "In order to investigate the dynamic strength of the interaction between lung surfactant protein D (SP-D) and different sugars, maltose, mannose, glucose, and galactose, we have used an atomic force microscope to monitor the interaction on a single molecule scale. The experiment is performed by measuring the rupture force when the SP-D-sugar bond is subjected to a continuously increasing force. Under these dynamic conditions, SP-D binds strongest to d-mannose and weakest to maltose and d-galactose. These results differ from equilibrium measurements wherein SP-D exhibits preference for maltose. On the basis of this finding, we propose that the binding of the disaccharide maltose to SP-D, which is energetically stronger than the binding of any of the monosacchrides, alters the structure of the binding site in a way that lowers the dynamic strength of the bond. We conclude that determining the strength of a protein-ligand bond under dynamic stress using an atomic force microscope is possibly more relevant for mimicking the actual nonequilibrium physiological situation in the lungs.",
author = "Esben Thormann and Dreyer, {Jakob K} and Simonsen, {Adam C} and Hansen, {Per Lyngs} and S{\o}ren Hansen and Uffe Holmskov and Mouritsen, {Ole G}",
note = "Keywords: Carbohydrates; Chromatography, Affinity; Chromatography, Ion Exchange; Enzyme-Linked Immunosorbent Assay; Ligands; Lung; Microscopy, Atomic Force; Protein Binding; Pulmonary Surfactant-Associated Protein D",
year = "2007",
doi = "10.1021/bi700823k",
language = "English",
volume = "46",
pages = "12231--7",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "43",
}