Dried urine swabs as a tool for monitoring metabolite excretion

Rastislav Monošík; Lars Ove Dragsted

doi:10.4155/bio-2018-0042

Dried urine swabs as a tool for monitoring metabolite excretion

Rastislav Monošík, Lars Ove Dragsted

Ernæring og Sundhed

6 Citationer (Scopus)

Abstract

Aim: We tested a large set (n = 181) of wet urine and dried urine samples spotted on regular cosmetic cotton swabs for comparative UHPLC-MSMS analysis of various metabolites across a wide polarity and structural range.

Results/methodology: The agreement of measurements between conventional 24 h urines and dried urine spots made from them in situ was evaluated by Passing-Bablok regression and Bland-Altman analysis after creatinine correction. There was full agreement in qualitative results but quantitative analysis revealed underestimation of dried urine spots in some cases. The dried urine samples contained analytes at measurable levels for at least 9 months.

Conclusion: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.

Originalsprog	Engelsk
Tidsskrift	Bioanalysis
Vol/bind	10
Udgave nummer	17
Sider (fra-til)	1371-1382
Antal sider	12
ISSN	1757-6180
DOI	https://doi.org/10.4155/bio-2018-0042
Status	Udgivet - 2018

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Det Natur- og Biovidenskabelige Fakultet

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10.4155/bio-2018-0042Licens: CC BY-NC-ND

bio-2018-0042Forlagets udgivne version, 3,98 MBLicens: CC BY-NC-ND
bio-2018-0042Forlagets udgivne version, 3,98 MBLicens: CC BY-NC-ND

Citationsformater

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author = "Rastislav Mono{\v s}{\'i}k and Dragsted, {Lars Ove}",

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AU - Dragsted, Lars Ove

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N2 - Aim: We tested a large set (n = 181) of wet urine and dried urine samples spotted on regular cosmetic cotton swabs for comparative UHPLC-MSMS analysis of various metabolites across a wide polarity and structural range. Results/methodology: The agreement of measurements between conventional 24 h urines and dried urine spots made from them in situ was evaluated by Passing-Bablok regression and Bland-Altman analysis after creatinine correction. There was full agreement in qualitative results but quantitative analysis revealed underestimation of dried urine spots in some cases. The dried urine samples contained analytes at measurable levels for at least 9 months.Conclusion: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.

AB - Aim: We tested a large set (n = 181) of wet urine and dried urine samples spotted on regular cosmetic cotton swabs for comparative UHPLC-MSMS analysis of various metabolites across a wide polarity and structural range. Results/methodology: The agreement of measurements between conventional 24 h urines and dried urine spots made from them in situ was evaluated by Passing-Bablok regression and Bland-Altman analysis after creatinine correction. There was full agreement in qualitative results but quantitative analysis revealed underestimation of dried urine spots in some cases. The dried urine samples contained analytes at measurable levels for at least 9 months.Conclusion: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.

KW - Faculty of Science

KW - UHPLC-MS/MS

KW - Creatinine correction

KW - Dried urine spots

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SP - 1371

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JO - Bioanalysis

JF - Bioanalysis

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Dried urine swabs as a tool for monitoring metabolite excretion

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