Dominant petroleum hydrocarbon-degrading bacteria in the archipelago sea in the South-West Finland (Baltic Sea) belong to different taxonomic groups than hydrocarbon degraders in the oceans

TY - JOUR

T1 - Dominant petroleum hydrocarbon-degrading bacteria in the archipelago sea in the South-West Finland (Baltic Sea) belong to different taxonomic groups than hydrocarbon degraders in the oceans

AU - Reunamo, Anna

AU - Riemann, Lasse

AU - Leskinen, Piia

AU - Jørgensen, Kirsten S

PY - 2013/7/15

Y1 - 2013/7/15

N2 - The natural petroleum hydrocarbon degrading capacity of the Archipelago Sea water in S-W Finland was studied in a microcosm experiment. Pristine and previously oil exposed sites were examined. Bacterial community fingerprinting was performed using terminal restriction fragment length polymorphism (T-RFLP) and samples from selected microcosms were sequenced. The abundance of PAH degradation genes was measured by quantitative PCR. Bacterial communities in diesel exposed microcosms diverged from control microcosms during the experiment. Gram positive PAH degradation genes dominated at both sites in situ, whereas gram negative PAH degrading genes became enriched in diesel microcosms. The dominant bacterial groups after a 14 days of diesel exposure were different depending on the sampling site, belonging to the class Actinobacteria (32%) at a pristine site and Betaproteobacteria (52%) at a previously oil exposed site. The hydrocarbon degrading bacteria in the Baltic Sea differ from those in the oceans, where most hydrocarbon degraders belong to Gammaproteobacteria.

AB - The natural petroleum hydrocarbon degrading capacity of the Archipelago Sea water in S-W Finland was studied in a microcosm experiment. Pristine and previously oil exposed sites were examined. Bacterial community fingerprinting was performed using terminal restriction fragment length polymorphism (T-RFLP) and samples from selected microcosms were sequenced. The abundance of PAH degradation genes was measured by quantitative PCR. Bacterial communities in diesel exposed microcosms diverged from control microcosms during the experiment. Gram positive PAH degradation genes dominated at both sites in situ, whereas gram negative PAH degrading genes became enriched in diesel microcosms. The dominant bacterial groups after a 14 days of diesel exposure were different depending on the sampling site, belonging to the class Actinobacteria (32%) at a pristine site and Betaproteobacteria (52%) at a previously oil exposed site. The hydrocarbon degrading bacteria in the Baltic Sea differ from those in the oceans, where most hydrocarbon degraders belong to Gammaproteobacteria.

U2 - 10.1016/j.marpolbul.2013.04.006

DO - 10.1016/j.marpolbul.2013.04.006

M3 - Journal article

C2 - 23711839

SN - 0025-326X

VL - 72

SP - 174

EP - 180

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 1

ER -