DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters

TY - JOUR

T1 - DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters

AU - Reinstrup, Line

AU - Jørgensen, Aslak

AU - Vennervald, Birgitte J.

AU - Kristensen, Thomas K.

PY - 2012/4

Y1 - 2012/4

N2 - Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.

AB - Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.

KW - Former LIFE faculty

KW - Schistosoma haematobium

KW - DNA extraction

KW - Egg

KW - Urine filtration

KW - Nylon filters

KW - Genetic variation

U2 - 10.1016/j.trstmh.2011.12.002

DO - 10.1016/j.trstmh.2011.12.002

M3 - Letter

C2 - 22381628

SN - 0035-9203

VL - 106

SP - 270

EP - 272

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 4

ER -