Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

Rupali Vohra; Iswariyaraja Sridevi Gurubaran; Ulrik  Henriksen; Linda Hildegaard Bergersen; Lene Juel Rasmussen; Claus Desler; Dorte Marie Skytt; Miriam Kolko

doi:10.1016/j.mito.2017.02.003

Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

Rupali Vohra, Iswariyaraja Sridevi Gurubaran, Ulrik Henriksen, Linda Hildegaard Bergersen, Lene Juel Rasmussen, Claus Desler, Dorte Marie Skytt, Miriam Kolko^*

^*Corresponding author af dette arbejde

10 Citationer (Scopus)

Abstract

Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.

Originalsprog	Engelsk
Tidsskrift	Mitochondrion
Vol/bind	36
Sider (fra-til)	52-59
ISSN	1567-7249
DOI	https://doi.org/10.1016/j.mito.2017.02.003
Status	Udgivet - sep. 2017

Adgang til dokumentet

10.1016/j.mito.2017.02.003

Citationsformater

@article{fae0ff39729e4d93b518b856c2b94c0e,

title = "Disturbed mitochondrial function restricts glutamate uptake in the human M{\"u}ller glia cell line, MIO-M1",

abstract = "Using the human M{\"u}ller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in M{\"u}ller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.",

keywords = "Antimycin A, Glutamate, Lactate, MIO-M1 cell line, Mitochondria, M{\"u}ller cells",

author = "Rupali Vohra and Gurubaran, {Iswariyaraja Sridevi} and Ulrik Henriksen and Bergersen, {Linda Hildegaard} and Rasmussen, {Lene Juel} and Claus Desler and Skytt, {Dorte Marie} and Miriam Kolko",

year = "2017",

month = sep,

doi = "10.1016/j.mito.2017.02.003",

language = "English",

volume = "36",

pages = "52--59",

journal = "Mitochondrion",

issn = "1567-7249",

publisher = "Elsevier",

}

TY - JOUR

T1 - Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

AU - Vohra, Rupali

AU - Gurubaran, Iswariyaraja Sridevi

AU - Henriksen, Ulrik

AU - Bergersen, Linda Hildegaard

AU - Rasmussen, Lene Juel

AU - Desler, Claus

AU - Skytt, Dorte Marie

AU - Kolko, Miriam

PY - 2017/9

Y1 - 2017/9

N2 - Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.

AB - Using the human Müller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in Müller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were studied in response to mitochondrial inhibition and glucose restriction. Lactate release decreased in response to glucose restriction. Combined glucose restriction and blocked mitochondrial activity decreased survival and caused collapse of the respiratory chain measured by oxygen consumption rate and extracellular acidification rate. Mitochondrial inhibition caused impaired glutamate uptake and decreased mRNA expression of the glutamate transporter, EAAT1. Over all, we show important roles of mitochondrial activity in MIO-M1 cell function and survival.

KW - Antimycin A

KW - Glutamate

KW - Lactate

KW - MIO-M1 cell line

KW - Mitochondria

KW - Müller cells

U2 - 10.1016/j.mito.2017.02.003

DO - 10.1016/j.mito.2017.02.003

M3 - Journal article

C2 - 28185966

AN - SCOPUS:85013149692

SN - 1567-7249

VL - 36

SP - 52

EP - 59

JO - Mitochondrion

JF - Mitochondrion

ER -

Disturbed mitochondrial function restricts glutamate uptake in the human Müller glia cell line, MIO-M1

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater