Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006

Jenny J Zhang; Tharanga N Senaratne; Rachel Daniels; Clarissa Valim; Michael Alifrangis; Priyanie Amerasinghe; Flemming Konradsen; Rupika Rajakaruna; Dyann F Wirth; Nadira D Karunaweera

doi:10.4269/ajtmh.2011.11-0167

Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006

Jenny J Zhang, Tharanga N Senaratne, Rachel Daniels, Clarissa Valim, Michael Alifrangis, Priyanie Amerasinghe, Flemming Konradsen, Rupika Rajakaruna, Dyann F Wirth, Nadira D Karunaweera

3 Citationer (Scopus)

Abstract

Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter ( pfcrt ; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency ( P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.

Originalsprog	Engelsk
Tidsskrift	American Journal of Tropical Medicine and Hygiene
Vol/bind	85
Udgave nummer	5
Sider (fra-til)	811-4
Antal sider	4
ISSN	0002-9637
DOI	https://doi.org/10.4269/ajtmh.2011.11-0167
Status	Udgivet - 1 nov. 2011

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Zhang, J. J., Senaratne, T. N., Daniels, R., Valim, C., Alifrangis, M., Amerasinghe, P., Konradsen, F., Rajakaruna, R., Wirth, D. F., & Karunaweera, N. D. (2011). Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006. American Journal of Tropical Medicine and Hygiene, 85(5), 811-4. https://doi.org/10.4269/ajtmh.2011.11-0167

Zhang, JJ, Senaratne, TN, Daniels, R, Valim, C, Alifrangis, M, Amerasinghe, P, Konradsen, F, Rajakaruna, R, Wirth, DF & Karunaweera, ND 2011, 'Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006', American Journal of Tropical Medicine and Hygiene, bind 85, nr. 5, s. 811-4. https://doi.org/10.4269/ajtmh.2011.11-0167

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title = "Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006",

abstract = "Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter ( pfcrt ; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency ( P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.",

author = "Zhang, {Jenny J} and Senaratne, {Tharanga N} and Rachel Daniels and Clarissa Valim and Michael Alifrangis and Priyanie Amerasinghe and Flemming Konradsen and Rupika Rajakaruna and Wirth, {Dyann F} and Karunaweera, {Nadira D}",

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doi = "10.4269/ajtmh.2011.11-0167",

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AU - Zhang, Jenny J

AU - Senaratne, Tharanga N

AU - Daniels, Rachel

AU - Valim, Clarissa

AU - Alifrangis, Michael

AU - Amerasinghe, Priyanie

AU - Konradsen, Flemming

AU - Rajakaruna, Rupika

AU - Wirth, Dyann F

AU - Karunaweera, Nadira D

PY - 2011/11/1

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N2 - Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter ( pfcrt ; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency ( P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.

AB - Widespread antimalarial resistance has been a barrier to malaria elimination efforts in Sri Lanka. Analysis of genetic markers in historic parasites may uncover trends in the spread of resistance. We examined the frequency of Plasmodium falciparum chloroquine transporter ( pfcrt ; codons 72-76) haplotypes in Sri Lanka in 1996-1998 and 2004-2006 using a high-resolution melting assay. Among 59 samples from 1996 to 1998, we detected the SVMNT (86%), CVMNK (10%), and CVIET (2%) haplotypes, with a positive trend in SVMNT and a negative trend in CVMNK frequency ( P = 0.004) over time. Among 24 samples from 2004 to 2006, we observed only the SVMNT haplotype. This finding indicates selection for the SVMNT haplotype over time and its possible fixation in the population.

U2 - 10.4269/ajtmh.2011.11-0167

DO - 10.4269/ajtmh.2011.11-0167

M3 - Journal article

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SN - 0002-9637

VL - 85

SP - 811

EP - 814

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

IS - 5

ER -

Distribution pattern of Plasmodium falciparum chloroquine transporter (pfcrt) gene haplotypes in Sri Lanka 1996-2006

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