Distinct expression of muscle-specific microRNAs (myomirs) in brown adipocytes

TY - JOUR

T1 - Distinct expression of muscle-specific microRNAs (myomirs) in brown adipocytes

AU - Walden, Tomas B

AU - Timmons, James A

AU - Keller, Pernille

AU - Nedergaard, Jan

AU - Cannon, Barbara

N1 - Keywords: Adipocytes, Brown; Adipogenesis; Animals; Biological Markers; Cell Line; Cell Lineage; Gene Expression Regulation; Male; Mice; MicroRNAs; Muscle Development; Muscle, Skeletal; Organ Specificity; RNA, Messenger

PY - 2009

Y1 - 2009

N2 - MicroRNAs, a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. Here we examine murine white and brown primary cell cultures for differential expression of miRNAs. The adipogenesis-related miRNA miR-143 was highly expressed in mature white adipocytes but was low in mature brown adipocytes. Three classical "myogenic" miRNAs miR-1, miR-133a and miR-206 were absent from white adipocytes but were specifically expressed both in brown pre- and mature adipocytes, reinforcing the concept that brown adipocytes and myocytes derive from a common cell lineage that specifies energy-dissipating cells. Augmentation of adipocyte differentiation status with norepinephrine or rosiglitazone did not affect the expression of the above miRNAs, the expression levels of which were thus innately regulated. However, expression of the miRNA miR-455 was enhanced during brown adipocyte differentiation, similarly to the expression pattern of the brown adipocyte differentiation marker UCP1. In conclusion, miRNAs are differentially expressed in white and brown adipocytes and may be important in defining the common precursor cell for myocytes and brown adipocytes and thus have distinct roles in energy-storing versus energy-dissipating cells.

AB - MicroRNAs, a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. Here we examine murine white and brown primary cell cultures for differential expression of miRNAs. The adipogenesis-related miRNA miR-143 was highly expressed in mature white adipocytes but was low in mature brown adipocytes. Three classical "myogenic" miRNAs miR-1, miR-133a and miR-206 were absent from white adipocytes but were specifically expressed both in brown pre- and mature adipocytes, reinforcing the concept that brown adipocytes and myocytes derive from a common cell lineage that specifies energy-dissipating cells. Augmentation of adipocyte differentiation status with norepinephrine or rosiglitazone did not affect the expression of the above miRNAs, the expression levels of which were thus innately regulated. However, expression of the miRNA miR-455 was enhanced during brown adipocyte differentiation, similarly to the expression pattern of the brown adipocyte differentiation marker UCP1. In conclusion, miRNAs are differentially expressed in white and brown adipocytes and may be important in defining the common precursor cell for myocytes and brown adipocytes and thus have distinct roles in energy-storing versus energy-dissipating cells.

U2 - 10.1002/jcp.21621

DO - 10.1002/jcp.21621

M3 - Journal article

C2 - 18937285

SN - 0021-9541

VL - 218

SP - 444

EP - 449

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 2

ER -