Disruption of the myostatin gene in porcine primary fibroblasts and embryos using zinc-finger nucleases

TY - JOUR

T1 - Disruption of the myostatin gene in porcine primary fibroblasts and embryos using zinc-finger nucleases

AU - Huang, Xian-Ju

AU - Zhang, Hong-Xiao

AU - Wang, Huili

AU - Xiong, Kai

AU - Qin, Ling

AU - Liu, Honglin

PY - 2014/4

Y1 - 2014/4

N2 - Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin lossof- function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

AB - Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin lossof- function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

U2 - 10.14348/molcells.2014.2209

DO - 10.14348/molcells.2014.2209

M3 - Journal article

C2 - 24802055

SN - 1016-8478

VL - 37

SP - 302

EP - 306

JO - Molecules and Cells

JF - Molecules and Cells

IS - 4

ER -