Discovery of novel algae-degrading enzymes from marine bacteria

Mikkel Schultz-Johansen; Pernille Kjersgaard Bech; Rosanna Catherine Hennessy; Peter Stougaard

Discovery of novel algae-degrading enzymes from marine bacteria

Mikkel Schultz-Johansen, Pernille Kjersgaard Bech, Rosanna Catherine Hennessy, Peter Stougaard

Microbial Ecology and Biotechnology

Abstract

Algal cell wall polysaccharides, and their derived oligosaccharides, display a range of health beneficial bioactive properties. Enzymes capable of degrading algal polysaccharides into oligosaccharides may be used to produce biomolecules with new functionalities for the food and pharma industry. Some marine bacteria are specialized in degrading algal biomass and secrete enzymes that can decompose the complex algal cell wall polysaccharides. In order to identify such bacteria and enzymatic activities, we have used a combination of traditional cultivation and isolation methods, bioinformatics and functional screening. This resulted in the discovery of a novel marine bacterium which displays a large enzymatic potential for degradation of red algal polysaccharides e.g. agar and carrageenan. In addition, we searched metagenome sequence data and identified new enzyme candidates for degradation of fucoidan. Enzyme-encoding genes were expressed in E.coli and functionally characterized.

Originalsprog	Engelsk
Publikationsdato	2017
Status	Udgivet - 2017
Begivenhed	Bioprosp_2017 : The 8th International Conference on Marine Biotechnology - Tromsø, Norge Varighed: 8 mar. 2017 → 10 mar. 2017 http://bioprosp.com/

Konference

Konference	Bioprosp_2017
Land/Område	Norge
By	Tromsø
Periode	08/03/2017 → 10/03/2017
Internetadresse	http://bioprosp.com/

FN’s Verdensmål

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Citationsformater

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title = "Discovery of novel algae-degrading enzymes from marine bacteria",

abstract = "Algal cell wall polysaccharides, and their derived oligosaccharides, display a range of health beneficial bioactive properties. Enzymes capable of degrading algal polysaccharides into oligosaccharides may be used to produce biomolecules with new functionalities for the food and pharma industry. Some marine bacteria are specialized in degrading algal biomass and secrete enzymes that can decompose the complex algal cell wall polysaccharides. In order to identify such bacteria and enzymatic activities, we have used a combination of traditional cultivation and isolation methods, bioinformatics and functional screening. This resulted in the discovery of a novel marine bacterium which displays a large enzymatic potential for degradation of red algal polysaccharides e.g. agar and carrageenan. In addition, we searched metagenome sequence data and identified new enzyme candidates for degradation of fucoidan. Enzyme-encoding genes were expressed in E.coli and functionally characterized. ",

author = "Mikkel Schultz-Johansen and Bech, {Pernille Kjersgaard} and Hennessy, {Rosanna Catherine} and Peter Stougaard",

year = "2017",

language = "English",

note = "Bioprosp_2017 : The 8th International Conference on Marine Biotechnology ; Conference date: 08-03-2017 Through 10-03-2017",

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TY - CONF

T1 - Discovery of novel algae-degrading enzymes from marine bacteria

AU - Schultz-Johansen, Mikkel

AU - Bech, Pernille Kjersgaard

AU - Hennessy, Rosanna Catherine

AU - Stougaard, Peter

PY - 2017

Y1 - 2017

N2 - Algal cell wall polysaccharides, and their derived oligosaccharides, display a range of health beneficial bioactive properties. Enzymes capable of degrading algal polysaccharides into oligosaccharides may be used to produce biomolecules with new functionalities for the food and pharma industry. Some marine bacteria are specialized in degrading algal biomass and secrete enzymes that can decompose the complex algal cell wall polysaccharides. In order to identify such bacteria and enzymatic activities, we have used a combination of traditional cultivation and isolation methods, bioinformatics and functional screening. This resulted in the discovery of a novel marine bacterium which displays a large enzymatic potential for degradation of red algal polysaccharides e.g. agar and carrageenan. In addition, we searched metagenome sequence data and identified new enzyme candidates for degradation of fucoidan. Enzyme-encoding genes were expressed in E.coli and functionally characterized.

AB - Algal cell wall polysaccharides, and their derived oligosaccharides, display a range of health beneficial bioactive properties. Enzymes capable of degrading algal polysaccharides into oligosaccharides may be used to produce biomolecules with new functionalities for the food and pharma industry. Some marine bacteria are specialized in degrading algal biomass and secrete enzymes that can decompose the complex algal cell wall polysaccharides. In order to identify such bacteria and enzymatic activities, we have used a combination of traditional cultivation and isolation methods, bioinformatics and functional screening. This resulted in the discovery of a novel marine bacterium which displays a large enzymatic potential for degradation of red algal polysaccharides e.g. agar and carrageenan. In addition, we searched metagenome sequence data and identified new enzyme candidates for degradation of fucoidan. Enzyme-encoding genes were expressed in E.coli and functionally characterized.

M3 - Poster

T2 - Bioprosp_2017

Y2 - 8 March 2017 through 10 March 2017

ER -

Discovery of novel algae-degrading enzymes from marine bacteria

Abstract

Konference

FN’s Verdensmål

Fingeraftryk

Citationsformater