Discovering novel hydrolases from hot environments

Roland Wohlgemuth; Jennifer Littlechild; Daniela Monti; Kirk Schnorr; Teunke van Rossum; Bettina Siebers; Peter Menzel; Ilya V. Kublanov; Anne Gunn Rike; Georgios Skretas; Zalan Szabo; Xu Peng; Mark J. Young

doi:10.1016/j.biotechadv.2018.09.004

Discovering novel hydrolases from hot environments

Roland Wohlgemuth^*, Jennifer Littlechild, Daniela Monti, Kirk Schnorr, Teunke van Rossum, Bettina Siebers, Peter Menzel, Ilya V. Kublanov, Anne Gunn Rike, Georgios Skretas, Zalan Szabo, Xu Peng, Mark J. Young

^*Corresponding author af dette arbejde

19 Citationer (Scopus)

Abstract

Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

Originalsprog	Engelsk
Tidsskrift	Biotechnology Advances
Vol/bind	36
Udgave nummer	8
Sider (fra-til)	2077-2100
ISSN	0734-9750
DOI	https://doi.org/10.1016/j.biotechadv.2018.09.004
Status	Udgivet - dec. 2018

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10.1016/j.biotechadv.2018.09.004

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title = "Discovering novel hydrolases from hot environments",

abstract = "Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.",

keywords = "Biocatalysis, Enrichment, Enzyme characterization, Enzyme screening, Gene expression, Genomics, Hydrolases, Metagenomics, Sequencing, Thermophiles",

author = "Roland Wohlgemuth and Jennifer Littlechild and Daniela Monti and Kirk Schnorr and {van Rossum}, Teunke and Bettina Siebers and Peter Menzel and Kublanov, {Ilya V.} and Rike, {Anne Gunn} and Georgios Skretas and Zalan Szabo and Xu Peng and Young, {Mark J.}",

year = "2018",

month = dec,

doi = "10.1016/j.biotechadv.2018.09.004",

language = "English",

volume = "36",

pages = "2077--2100",

journal = "Biotechnology Advances",

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AU - Wohlgemuth, Roland

AU - Littlechild, Jennifer

AU - Monti, Daniela

AU - Schnorr, Kirk

AU - van Rossum, Teunke

AU - Siebers, Bettina

AU - Menzel, Peter

AU - Kublanov, Ilya V.

AU - Rike, Anne Gunn

AU - Skretas, Georgios

AU - Szabo, Zalan

AU - Peng, Xu

AU - Young, Mark J.

PY - 2018/12

Y1 - 2018/12

N2 - Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

AB - Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

KW - Biocatalysis

KW - Enrichment

KW - Enzyme characterization

KW - Enzyme screening

KW - Gene expression

KW - Genomics

KW - Hydrolases

KW - Metagenomics

KW - Sequencing

KW - Thermophiles

U2 - 10.1016/j.biotechadv.2018.09.004

DO - 10.1016/j.biotechadv.2018.09.004

M3 - Journal article

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SN - 0734-9750

VL - 36

SP - 2077

EP - 2100

JO - Biotechnology Advances

JF - Biotechnology Advances

IS - 8

ER -

Discovering novel hydrolases from hot environments

Abstract

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