TY - JOUR
T1 - Direct microcomputer controlled determination of zinc in human serum by flow injection atomic absorption spectrometry
AU - Simonsen, Kirsten Wiese
AU - Nielsen, Bent
AU - Jensen, Arne
AU - Andersen, Jan Rud
PY - 1986/1/1
Y1 - 1986/1/1
N2 - A procedure is described for the direct determination of zinc in human serum by fully automated, microcomputer controlled flow injection atomic absorption spectrometry (Fl-AAS). The Fl system is pumpless, using the negative pressure created by the nebuliser. It only consists of a three-way valve, programmable from the microcomputer, to control the sample volume. No pre-treatment of the samples is necessary. The limit of detection is 0.14 mg l–1, and only small amounts of serum (<150 µl) are needed for the analysis. The accuracy, as estimated by analysing sera with known amounts of zinc, is excellent, and the between batch precision is ca. 5% RSD. Peak-area measurements are used in the procedure; as a consequence the quantitation can be carried out by use of an aqueous calibration graph. The sample throughput is 40 h–1, making the procedure well suited for routine clinical analysis.
AB - A procedure is described for the direct determination of zinc in human serum by fully automated, microcomputer controlled flow injection atomic absorption spectrometry (Fl-AAS). The Fl system is pumpless, using the negative pressure created by the nebuliser. It only consists of a three-way valve, programmable from the microcomputer, to control the sample volume. No pre-treatment of the samples is necessary. The limit of detection is 0.14 mg l–1, and only small amounts of serum (<150 µl) are needed for the analysis. The accuracy, as estimated by analysing sera with known amounts of zinc, is excellent, and the between batch precision is ca. 5% RSD. Peak-area measurements are used in the procedure; as a consequence the quantitation can be carried out by use of an aqueous calibration graph. The sample throughput is 40 h–1, making the procedure well suited for routine clinical analysis.
KW - Former Faculty of Pharmaceutical Sciences
KW - Zinc determination
KW - Flow Injection
KW - flame atomic absorption spectrometry
KW - human serum
KW - microcomputer control
U2 - 10.1039/JA9860100453
DO - 10.1039/JA9860100453
M3 - Journal article
SN - 0267-9477
VL - 1
SP - 453
EP - 456
JO - Journal of Analytical Atomic Spectrometry
JF - Journal of Analytical Atomic Spectrometry
IS - 6
ER -