TY - JOUR
T1 - Differences in non-enzymatic glycation products in human dentine and clavicle
T2 - changes with aging
AU - Valenzuela, Aurora
AU - Guerra-Hernández, Eduardo
AU - Rufián-Henares, José Ángel
AU - Márquez-Ruiz, Ana Belén
AU - Hougen, Hans Petter
AU - García-Villanova, Belén
PY - 2018/11
Y1 - 2018/11
N2 - In recent decades, several methods based on biochemical and molecular changes caused by aging have been proposed to improve the accuracy of forensic age estimation. The present study aimed to measure changes in furosine and pentosidine, two markers of non-enzymatic glycation of proteins (NEGs), in human dentine and clavicle with aging, and to identify possible differences between turnover rates in different mineralized tissues. Furosine and pentosidine were quantified in 32 dentine samples from living donors between 14 and 80 years of age, and in a second group of samples consisting of a tooth and a piece of clavicle collected from the same cadaver (15 individuals aged 18 to 85 years). Furosine concentration was much higher than pentosidine concentration in the same tissue, although they were strongly correlated in both dentine and bone. A close relationship between furosine and/or pentosidine content and chronological age was found in both tissues (r > 0.93). Moreover, age estimation was more accurate when furosine or pentosidine content was determined in dentine, with specificity values for the tests higher than 82% in all age groups. In clavicle, furosine concentration and pentosidine concentration were much lower (2.6-fold and 3.1-fold, respectively) than in dentine from the same individuals. In conclusion, although the results show strong correlations between chronological age and furosine or pentosidine concentrations determined in mineralized tissues, there is still a need for further research with larger data sets, including patients with diabetes.
AB - In recent decades, several methods based on biochemical and molecular changes caused by aging have been proposed to improve the accuracy of forensic age estimation. The present study aimed to measure changes in furosine and pentosidine, two markers of non-enzymatic glycation of proteins (NEGs), in human dentine and clavicle with aging, and to identify possible differences between turnover rates in different mineralized tissues. Furosine and pentosidine were quantified in 32 dentine samples from living donors between 14 and 80 years of age, and in a second group of samples consisting of a tooth and a piece of clavicle collected from the same cadaver (15 individuals aged 18 to 85 years). Furosine concentration was much higher than pentosidine concentration in the same tissue, although they were strongly correlated in both dentine and bone. A close relationship between furosine and/or pentosidine content and chronological age was found in both tissues (r > 0.93). Moreover, age estimation was more accurate when furosine or pentosidine content was determined in dentine, with specificity values for the tests higher than 82% in all age groups. In clavicle, furosine concentration and pentosidine concentration were much lower (2.6-fold and 3.1-fold, respectively) than in dentine from the same individuals. In conclusion, although the results show strong correlations between chronological age and furosine or pentosidine concentrations determined in mineralized tissues, there is still a need for further research with larger data sets, including patients with diabetes.
KW - Age estimation
KW - Forensic sciences
KW - Furosine
KW - Human bone
KW - Human dentine
KW - Pentosidine
KW - Aging/metabolism
KW - Humans
KW - Middle Aged
KW - Forensic Sciences
KW - Male
KW - Arginine/analogs & derivatives
KW - Clavicle/metabolism
KW - Biomarkers/metabolism
KW - Young Adult
KW - Adolescent
KW - Age Determination by Skeleton/methods
KW - Aged, 80 and over
KW - Adult
KW - Dentin/metabolism
KW - Female
KW - Lysine/analogs & derivatives
KW - Aged
KW - Age Determination by Teeth/methods
UR - http://www.scopus.com/inward/record.url?scp=85051250597&partnerID=8YFLogxK
U2 - 10.1007/s00414-018-1908-3
DO - 10.1007/s00414-018-1908-3
M3 - Journal article
C2 - 30069788
AN - SCOPUS:85051250597
SN - 0937-9827
VL - 132
SP - 1749
EP - 1758
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
IS - 6
ER -
