Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs

Ken Steen Pedersen; Helle Stege; Tim Kåre Jensen; Roberto Guedes; Marie Ståhl; Jens Peter Nielsen; Charlotte Kristiane Hjulsager; Lars Erik Larsen; Øystein Angen

doi:10.1177/1040638713480499

Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs

Ken Steen Pedersen, Helle Stege, Tim Kåre Jensen, Roberto Guedes, Marie Ståhl, Jens Peter Nielsen, Charlotte Kristiane Hjulsager, Lars Erik Larsen, Øystein Angen

7 Citationer (Scopus)

Abstract

Quantitative polymerase chain reaction (qPCR) tests for detection and quantification of Lawsonia intracellularis in feces from pigs have been developed. The objective of the current study was to evaluate the diagnostic performance of a fecal qPCR test for detection of nursery pigs with L. intracellularis-associated proliferative enteropathy (PE) under field conditions. Furthermore, the diagnostic performance for different subpopulations of pigs was investigated, including pigs infected or noninfected with Porcine circovirus-2, Brachyspira pilosicoli, and Escherichia coli. The diagnostic performance was evaluated in terms of diagnostic sensitivity and specificity. Data from pigs originating from 20 herds with antibiotic treatment requiring diarrhea outbreaks from a prior study were reused. Before treatment, pigs were randomly selected for histological and immunohistochemical examination of intestinal segments and fecal quantification of L. intracellularis by qPCR. A total of 313 pigs (157 without diarrhea, 156 with diarrhea) were included in the statistical analysis, and 37 pigs (11.8%) were classified as PE positives (defined as proliferative histological lesions in combination with L. intracellularis demonstration by immunohistochemistry). Lawsonia intracellularis was detected by qPCR in feces from 91 pigs (29.1%). A nonparametric receiver operating characteristic (ROC) analysis provided an area under the ROC curve (0.93) and an optimal cutoff value of 4.8 log₁₀L. intracellularis bacteria/g feces. This cutoff provided a diagnostic sensitivity of 0.84 and diagnostic specificity of 0.93. The diagnostic sensitivity and specificity were significantly different between herds (P < 0.0001). Numerically, diagnostic sensitivity and specificity were different between subpopulations of pigs, but no significant differences were demonstrated.

Originalsprog	Engelsk
Tidsskrift	Journal of Veterinary Diagnostic Investigation
Vol/bind	25
Udgave nummer	3
Sider (fra-til)	336-340
Antal sider	5
ISSN	1040-6387
DOI	https://doi.org/10.1177/1040638713480499
Status	Udgivet - maj 2013

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10.1177/1040638713480499

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Pedersen, K. S., Stege, H., Jensen, T. K., Guedes, R., Ståhl, M., Nielsen, J. P., Hjulsager, C. K., Larsen, L. E., & Angen, Ø. (2013). Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs. Journal of Veterinary Diagnostic Investigation, 25(3), 336-340. https://doi.org/10.1177/1040638713480499

Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs. / Pedersen, Ken Steen ; Stege, Helle; Jensen, Tim Kåre et al.
I: Journal of Veterinary Diagnostic Investigation, Bind 25, Nr. 3, 05.2013, s. 336-340.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Pedersen, KS , Stege, H, Jensen, TK, Guedes, R, Ståhl, M, Nielsen, JP, Hjulsager, CK, Larsen, LE & Angen, Ø 2013, 'Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs', Journal of Veterinary Diagnostic Investigation, bind 25, nr. 3, s. 336-340. https://doi.org/10.1177/1040638713480499

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title = "Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs",

abstract = "Quantitative polymerase chain reaction (qPCR) tests for detection and quantification of Lawsonia intracellularis in feces from pigs have been developed. The objective of the current study was to evaluate the diagnostic performance of a fecal qPCR test for detection of nursery pigs with L. intracellularis-associated proliferative enteropathy (PE) under field conditions. Furthermore, the diagnostic performance for different subpopulations of pigs was investigated, including pigs infected or noninfected with Porcine circovirus-2, Brachyspira pilosicoli, and Escherichia coli. The diagnostic performance was evaluated in terms of diagnostic sensitivity and specificity. Data from pigs originating from 20 herds with antibiotic treatment requiring diarrhea outbreaks from a prior study were reused. Before treatment, pigs were randomly selected for histological and immunohistochemical examination of intestinal segments and fecal quantification of L. intracellularis by qPCR. A total of 313 pigs (157 without diarrhea, 156 with diarrhea) were included in the statistical analysis, and 37 pigs (11.8%) were classified as PE positives (defined as proliferative histological lesions in combination with L. intracellularis demonstration by immunohistochemistry). Lawsonia intracellularis was detected by qPCR in feces from 91 pigs (29.1%). A nonparametric receiver operating characteristic (ROC) analysis provided an area under the ROC curve (0.93) and an optimal cutoff value of 4.8 log10L. intracellularis bacteria/g feces. This cutoff provided a diagnostic sensitivity of 0.84 and diagnostic specificity of 0.93. The diagnostic sensitivity and specificity were significantly different between herds (P < 0.0001). Numerically, diagnostic sensitivity and specificity were different between subpopulations of pigs, but no significant differences were demonstrated.",

author = "Pedersen, {Ken Steen} and Helle Stege and Jensen, {Tim K{\aa}re} and Roberto Guedes and Marie St{\aa}hl and Nielsen, {Jens Peter} and Hjulsager, {Charlotte Kristiane} and Larsen, {Lars Erik} and {\O}ystein Angen",

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AU - Pedersen, Ken Steen

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AU - Jensen, Tim Kåre

AU - Guedes, Roberto

AU - Ståhl, Marie

AU - Nielsen, Jens Peter

AU - Hjulsager, Charlotte Kristiane

AU - Larsen, Lars Erik

AU - Angen, Øystein

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N2 - Quantitative polymerase chain reaction (qPCR) tests for detection and quantification of Lawsonia intracellularis in feces from pigs have been developed. The objective of the current study was to evaluate the diagnostic performance of a fecal qPCR test for detection of nursery pigs with L. intracellularis-associated proliferative enteropathy (PE) under field conditions. Furthermore, the diagnostic performance for different subpopulations of pigs was investigated, including pigs infected or noninfected with Porcine circovirus-2, Brachyspira pilosicoli, and Escherichia coli. The diagnostic performance was evaluated in terms of diagnostic sensitivity and specificity. Data from pigs originating from 20 herds with antibiotic treatment requiring diarrhea outbreaks from a prior study were reused. Before treatment, pigs were randomly selected for histological and immunohistochemical examination of intestinal segments and fecal quantification of L. intracellularis by qPCR. A total of 313 pigs (157 without diarrhea, 156 with diarrhea) were included in the statistical analysis, and 37 pigs (11.8%) were classified as PE positives (defined as proliferative histological lesions in combination with L. intracellularis demonstration by immunohistochemistry). Lawsonia intracellularis was detected by qPCR in feces from 91 pigs (29.1%). A nonparametric receiver operating characteristic (ROC) analysis provided an area under the ROC curve (0.93) and an optimal cutoff value of 4.8 log10L. intracellularis bacteria/g feces. This cutoff provided a diagnostic sensitivity of 0.84 and diagnostic specificity of 0.93. The diagnostic sensitivity and specificity were significantly different between herds (P < 0.0001). Numerically, diagnostic sensitivity and specificity were different between subpopulations of pigs, but no significant differences were demonstrated.

AB - Quantitative polymerase chain reaction (qPCR) tests for detection and quantification of Lawsonia intracellularis in feces from pigs have been developed. The objective of the current study was to evaluate the diagnostic performance of a fecal qPCR test for detection of nursery pigs with L. intracellularis-associated proliferative enteropathy (PE) under field conditions. Furthermore, the diagnostic performance for different subpopulations of pigs was investigated, including pigs infected or noninfected with Porcine circovirus-2, Brachyspira pilosicoli, and Escherichia coli. The diagnostic performance was evaluated in terms of diagnostic sensitivity and specificity. Data from pigs originating from 20 herds with antibiotic treatment requiring diarrhea outbreaks from a prior study were reused. Before treatment, pigs were randomly selected for histological and immunohistochemical examination of intestinal segments and fecal quantification of L. intracellularis by qPCR. A total of 313 pigs (157 without diarrhea, 156 with diarrhea) were included in the statistical analysis, and 37 pigs (11.8%) were classified as PE positives (defined as proliferative histological lesions in combination with L. intracellularis demonstration by immunohistochemistry). Lawsonia intracellularis was detected by qPCR in feces from 91 pigs (29.1%). A nonparametric receiver operating characteristic (ROC) analysis provided an area under the ROC curve (0.93) and an optimal cutoff value of 4.8 log10L. intracellularis bacteria/g feces. This cutoff provided a diagnostic sensitivity of 0.84 and diagnostic specificity of 0.93. The diagnostic sensitivity and specificity were significantly different between herds (P < 0.0001). Numerically, diagnostic sensitivity and specificity were different between subpopulations of pigs, but no significant differences were demonstrated.

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Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis−associated proliferative enteropathy in nursery pigs

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