Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria

Ida Nordang Kieler; Melania Osto; Leoni Hugentobler; Lara Christine Puetz; Tom Gilbert; Torben Hansen; Oluf Borbye Pedersen; Claudia E. Reusch; Eric  Zini; Thomas A Lutz; Charlotte Reinhard Bjørnvad

doi:10.1038/s41598-019-41195-0

Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria

Ida Nordang Kieler, Melania Osto, Leoni Hugentobler, Lara Christine Puetz, Tom Gilbert, Torben Hansen, Oluf Borbye Pedersen, Claudia E. Reusch, Eric Zini, Thomas A Lutz, Charlotte Reinhard Bjørnvad

16 Citationer (Scopus)

32 Downloads (Pure)

Abstract

Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of the current study was firstly a cross-sectional comparison of the GM of diabetic cats, to that of lean, and of obese/overweight non-diabetic cats of a similar age. Specifically, fecal samples from 82 privately-owned cats from Denmark and Switzerland were sequenced using 16S rRNA gene amplicon metabarcoding. Secondly dietary intervention data was generated, by obtaining additional samples from a subset of cats after placing them on a high-protein diet for four weeks. The GM diversity of diabetic cats was lower than that of lean cats in the cross-sectional study, and lower compared to lean and to overweight/obese cats after diet intervention. Diabetic cats also exhibited fewer Anaerotruncus, Dialister, and unknown Ruminococcaceae than lean cats. Serum fructosamine levels correlated negatively with Prevotellaceae abundance and positively with Enterobacteriaceae abundance. In summary the intestinal microbiota of diabetic cats was characterized by decreased GM diversity and loss of butyrate producing bacterial genera.

Originalsprog	Engelsk
Artikelnummer	4822
Tidsskrift	Scientific Reports
Vol/bind	9
Sider (fra-til)	1-13
Antal sider	13
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-019-41195-0
Status	Udgivet - 1 dec. 2019

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10.1038/s41598-019-41195-0Licens: CC BY

s41598-019-41195-0Forlagets udgivne version, 1,96 MBLicens: CC BY

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title = "Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria",

abstract = "Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of the current study was firstly a cross-sectional comparison of the GM of diabetic cats, to that of lean, and of obese/overweight non-diabetic cats of a similar age. Specifically, fecal samples from 82 privately-owned cats from Denmark and Switzerland were sequenced using 16S rRNA gene amplicon metabarcoding. Secondly dietary intervention data was generated, by obtaining additional samples from a subset of cats after placing them on a high-protein diet for four weeks. The GM diversity of diabetic cats was lower than that of lean cats in the cross-sectional study, and lower compared to lean and to overweight/obese cats after diet intervention. Diabetic cats also exhibited fewer Anaerotruncus, Dialister, and unknown Ruminococcaceae than lean cats. Serum fructosamine levels correlated negatively with Prevotellaceae abundance and positively with Enterobacteriaceae abundance. In summary the intestinal microbiota of diabetic cats was characterized by decreased GM diversity and loss of butyrate producing bacterial genera.",

author = "Kieler, {Ida Nordang} and Melania Osto and Leoni Hugentobler and Puetz, {Lara Christine} and Tom Gilbert and Torben Hansen and Pedersen, {Oluf Borbye} and Reusch, {Claudia E.} and Eric Zini and Lutz, {Thomas A} and Bj{\o}rnvad, {Charlotte Reinhard}",

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T1 - Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria

AU - Kieler, Ida Nordang

AU - Osto, Melania

AU - Hugentobler, Leoni

AU - Puetz, Lara Christine

AU - Gilbert, Tom

AU - Hansen, Torben

AU - Pedersen, Oluf Borbye

AU - Reusch, Claudia E.

AU - Zini, Eric

AU - Lutz, Thomas A

AU - Bjørnvad, Charlotte Reinhard

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of the current study was firstly a cross-sectional comparison of the GM of diabetic cats, to that of lean, and of obese/overweight non-diabetic cats of a similar age. Specifically, fecal samples from 82 privately-owned cats from Denmark and Switzerland were sequenced using 16S rRNA gene amplicon metabarcoding. Secondly dietary intervention data was generated, by obtaining additional samples from a subset of cats after placing them on a high-protein diet for four weeks. The GM diversity of diabetic cats was lower than that of lean cats in the cross-sectional study, and lower compared to lean and to overweight/obese cats after diet intervention. Diabetic cats also exhibited fewer Anaerotruncus, Dialister, and unknown Ruminococcaceae than lean cats. Serum fructosamine levels correlated negatively with Prevotellaceae abundance and positively with Enterobacteriaceae abundance. In summary the intestinal microbiota of diabetic cats was characterized by decreased GM diversity and loss of butyrate producing bacterial genera.

AB - Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of the current study was firstly a cross-sectional comparison of the GM of diabetic cats, to that of lean, and of obese/overweight non-diabetic cats of a similar age. Specifically, fecal samples from 82 privately-owned cats from Denmark and Switzerland were sequenced using 16S rRNA gene amplicon metabarcoding. Secondly dietary intervention data was generated, by obtaining additional samples from a subset of cats after placing them on a high-protein diet for four weeks. The GM diversity of diabetic cats was lower than that of lean cats in the cross-sectional study, and lower compared to lean and to overweight/obese cats after diet intervention. Diabetic cats also exhibited fewer Anaerotruncus, Dialister, and unknown Ruminococcaceae than lean cats. Serum fructosamine levels correlated negatively with Prevotellaceae abundance and positively with Enterobacteriaceae abundance. In summary the intestinal microbiota of diabetic cats was characterized by decreased GM diversity and loss of butyrate producing bacterial genera.

U2 - 10.1038/s41598-019-41195-0

DO - 10.1038/s41598-019-41195-0

M3 - Journal article

C2 - 30886210

SN - 2045-2322

VL - 9

SP - 1

EP - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 4822

ER -

Diabetic cats have decreased gut microbial diversity and a lack of butyrate producing bacteria

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