Developmental changes of rRNA ribose methylations in the mouse

Jade Hebras; Nicolai Krogh; Virginie Marty; Henrik Nielsen; Jérôme Cavaillé

doi:10.1080/15476286.2019.1670598

Developmental changes of rRNA ribose methylations in the mouse

Jade Hebras, Nicolai Krogh, Virginie Marty, Henrik Nielsen, Jérôme Cavaillé

Transcription, RNA, and Gene Medicine Program

11 Citationer (Scopus)

Abstract

A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.

Originalsprog	Engelsk
Tidsskrift	RNA Biology
Sider (fra-til)	1-15
Antal sider	15
ISSN	1547-6286
DOI	https://doi.org/10.1080/15476286.2019.1670598
Status	Udgivet - 2 jan. 2020

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10.1080/15476286.2019.1670598

15476286.2019.1670598?needAccess=trueForlagets udgivne version, 4,17 MB

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title = "Developmental changes of rRNA ribose methylations in the mouse",

abstract = "A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.",

author = "Jade Hebras and Nicolai Krogh and Virginie Marty and Henrik Nielsen and J{\'e}r{\^o}me Cavaill{\'e}",

year = "2020",

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doi = "10.1080/15476286.2019.1670598",

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T1 - Developmental changes of rRNA ribose methylations in the mouse

AU - Hebras, Jade

AU - Krogh, Nicolai

AU - Marty, Virginie

AU - Nielsen, Henrik

AU - Cavaillé, Jérôme

PY - 2020/1/2

Y1 - 2020/1/2

N2 - A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.

AB - A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.

U2 - 10.1080/15476286.2019.1670598

DO - 10.1080/15476286.2019.1670598

M3 - Journal article

C2 - 31566069

SN - 1547-6286

SP - 1

EP - 15

JO - RNA Biology

JF - RNA Biology

ER -

Developmental changes of rRNA ribose methylations in the mouse

Abstract

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