Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation

Nathja Ravn; Nora Elisabeth Zois; Sophia G. Moesgaard; Jesper Langhoff Hønge; Morten Holdgaard Smerup; J. Michael Hasenkam; Erik Sloth; Signe Emilie Cremer; Lisbeth Høier Olsen

doi:10.3109/14017431.2014.895850

Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation

Nathja Ravn, Nora Elisabeth Zois, Sophia G. Moesgaard, Jesper Langhoff Hønge, Morten Holdgaard Smerup, J. Michael Hasenkam, Erik Sloth, Signe Emilie Cremer, Lisbeth Høier Olsen

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Abstract

Objectives. We aimed to develop a porcine model for chronic nonischemic mitral regurgitation (MR) to investigate left ventricular (LV) enlargement and eccentric hypertrophy. Design. Nonischemic MR was induced in 30 pigs by open-chest immobilization of the posterior mitral leaflet by transannular traction sutures that where applied in transmyocardial fashion. A sham operated control group (n = 13) was included. Echocardiographic LV size and heart weight assessed at euthanasia were used to evaluate the development of LV enlargement and eccentric hypertrophy after 8 weeks follow-up. Results. Eight pigs died and seven were excluded due to mediastinal infection (n = 2) or failure to produce MR (n = 5). Thus, 28 pigs were included and were divided into three groups: controls (n = 12), mild MR (mMR; n = 10), and moderate to severe MR (sMR; n = 6). The change in LV internal diameter in diastole (LVIDd) from baseline to follow-up was significantly higher in the sMR group compared to that of the control group (P = 0.0017). Furthermore, LV weight was significantly increased in the mMR (P = 0.047) and the sMR (P = 0.0087) groups compared to that of the control group. Conclusions. A new model for chronic moderate to severe nonischemic MR with development of LV enlargement and eccentric hypertrophy within 8 weeks has been established in pigs.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Cardiovascular Journal
Vol/bind	48
Udgave nummer	3
Sider (fra-til)	189-195
Antal sider	7
ISSN	1401-7431
DOI	https://doi.org/10.3109/14017431.2014.895850
Status	Udgivet - jun. 2014

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@article{b778959129654ad98832f5fa2677a988,

title = "Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation",

abstract = "Objectives. We aimed to develop a porcine model for chronic nonischemic mitral regurgitation (MR) to investigate left ventricular (LV) enlargement and eccentric hypertrophy. Design. Nonischemic MR was induced in 30 pigs by open-chest immobilization of the posterior mitral leaflet by transannular traction sutures that where applied in transmyocardial fashion. A sham operated control group (n = 13) was included. Echocardiographic LV size and heart weight assessed at euthanasia were used to evaluate the development of LV enlargement and eccentric hypertrophy after 8 weeks follow-up. Results. Eight pigs died and seven were excluded due to mediastinal infection (n = 2) or failure to produce MR (n = 5). Thus, 28 pigs were included and were divided into three groups: controls (n = 12), mild MR (mMR; n = 10), and moderate to severe MR (sMR; n = 6). The change in LV internal diameter in diastole (LVIDd) from baseline to follow-up was significantly higher in the sMR group compared to that of the control group (P = 0.0017). Furthermore, LV weight was significantly increased in the mMR (P = 0.047) and the sMR (P = 0.0087) groups compared to that of the control group. Conclusions. A new model for chronic moderate to severe nonischemic MR with development of LV enlargement and eccentric hypertrophy within 8 weeks has been established in pigs.",

keywords = "Faculty of Health and Medical Sciences, animal model, experimental, left ventricular hypertrophy, left ventricular remodeling, mitral valve incompetence",

author = "Nathja Ravn and Zois, {Nora Elisabeth} and Moesgaard, {Sophia G.} and H{\o}nge, {Jesper Langhoff} and Smerup, {Morten Holdgaard} and Hasenkam, {J. Michael} and Erik Sloth and Cremer, {Signe Emilie} and Olsen, {Lisbeth H{\o}ier}",

year = "2014",

month = jun,

doi = "10.3109/14017431.2014.895850",

language = "English",

volume = "48",

pages = "189--195",

journal = "Scandinavian Cardiovascular Journal",

issn = "1401-7431",

publisher = "Taylor & Francis",

number = "3",

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TY - JOUR

T1 - Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation

AU - Ravn, Nathja

AU - Zois, Nora Elisabeth

AU - Moesgaard, Sophia G.

AU - Hønge, Jesper Langhoff

AU - Smerup, Morten Holdgaard

AU - Hasenkam, J. Michael

AU - Sloth, Erik

AU - Cremer, Signe Emilie

AU - Olsen, Lisbeth Høier

PY - 2014/6

Y1 - 2014/6

N2 - Objectives. We aimed to develop a porcine model for chronic nonischemic mitral regurgitation (MR) to investigate left ventricular (LV) enlargement and eccentric hypertrophy. Design. Nonischemic MR was induced in 30 pigs by open-chest immobilization of the posterior mitral leaflet by transannular traction sutures that where applied in transmyocardial fashion. A sham operated control group (n = 13) was included. Echocardiographic LV size and heart weight assessed at euthanasia were used to evaluate the development of LV enlargement and eccentric hypertrophy after 8 weeks follow-up. Results. Eight pigs died and seven were excluded due to mediastinal infection (n = 2) or failure to produce MR (n = 5). Thus, 28 pigs were included and were divided into three groups: controls (n = 12), mild MR (mMR; n = 10), and moderate to severe MR (sMR; n = 6). The change in LV internal diameter in diastole (LVIDd) from baseline to follow-up was significantly higher in the sMR group compared to that of the control group (P = 0.0017). Furthermore, LV weight was significantly increased in the mMR (P = 0.047) and the sMR (P = 0.0087) groups compared to that of the control group. Conclusions. A new model for chronic moderate to severe nonischemic MR with development of LV enlargement and eccentric hypertrophy within 8 weeks has been established in pigs.

AB - Objectives. We aimed to develop a porcine model for chronic nonischemic mitral regurgitation (MR) to investigate left ventricular (LV) enlargement and eccentric hypertrophy. Design. Nonischemic MR was induced in 30 pigs by open-chest immobilization of the posterior mitral leaflet by transannular traction sutures that where applied in transmyocardial fashion. A sham operated control group (n = 13) was included. Echocardiographic LV size and heart weight assessed at euthanasia were used to evaluate the development of LV enlargement and eccentric hypertrophy after 8 weeks follow-up. Results. Eight pigs died and seven were excluded due to mediastinal infection (n = 2) or failure to produce MR (n = 5). Thus, 28 pigs were included and were divided into three groups: controls (n = 12), mild MR (mMR; n = 10), and moderate to severe MR (sMR; n = 6). The change in LV internal diameter in diastole (LVIDd) from baseline to follow-up was significantly higher in the sMR group compared to that of the control group (P = 0.0017). Furthermore, LV weight was significantly increased in the mMR (P = 0.047) and the sMR (P = 0.0087) groups compared to that of the control group. Conclusions. A new model for chronic moderate to severe nonischemic MR with development of LV enlargement and eccentric hypertrophy within 8 weeks has been established in pigs.

KW - Faculty of Health and Medical Sciences

KW - animal model

KW - experimental

KW - left ventricular hypertrophy

KW - left ventricular remodeling

KW - mitral valve incompetence

U2 - 10.3109/14017431.2014.895850

DO - 10.3109/14017431.2014.895850

M3 - Journal article

C2 - 24548173

SN - 1401-7431

VL - 48

SP - 189

EP - 195

JO - Scandinavian Cardiovascular Journal

JF - Scandinavian Cardiovascular Journal

IS - 3

ER -

Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation

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