Development of chromogenic true-to-nature biomass substrates for bioconversion process optimization

TY - GEN

T1 - Development of chromogenic true-to-nature biomass substrates for bioconversion process optimization

AU - Kračun, Stjepan K.

N1 - Conference code: 26

PY - 2018

Y1 - 2018

N2 - High-quality analysis of pretreatment outcome, cost-effective enzymatic hydrolysis and discovery of new efficient enzymes are needed in order to obtain commercially viable production methods for 2G bioethanol. GlycoSpot has already demonstrated that multi-colored chromogenic polysaccharide and protein substrates can be used in cheap, convenient and high-throughput multiplexed assays. In addition, we have produced substrates out of biomass materials in which the complexity of plant cell walls is partially maintained. These substrates can be used to screen the activities of different carbohydrate-acting enzymes and provide insight into substrate availability within biomass. However, these substrates do not allow screening for enzymes where the fine structure of the substrate is required -for example esterases and exo-acting enzymes. We aim to develop the next generation of biomass substrates in which native structure are minimally modified and chemical substitutions are maintained. These novel True-To-Nature Complex Biomaterial Substrates (TTN-CBS) would more closely mimic natural biomass encountered in biorefining and new findings can be directly applied to improvement of raw material utilization.

AB - High-quality analysis of pretreatment outcome, cost-effective enzymatic hydrolysis and discovery of new efficient enzymes are needed in order to obtain commercially viable production methods for 2G bioethanol. GlycoSpot has already demonstrated that multi-colored chromogenic polysaccharide and protein substrates can be used in cheap, convenient and high-throughput multiplexed assays. In addition, we have produced substrates out of biomass materials in which the complexity of plant cell walls is partially maintained. These substrates can be used to screen the activities of different carbohydrate-acting enzymes and provide insight into substrate availability within biomass. However, these substrates do not allow screening for enzymes where the fine structure of the substrate is required -for example esterases and exo-acting enzymes. We aim to develop the next generation of biomass substrates in which native structure are minimally modified and chemical substitutions are maintained. These novel True-To-Nature Complex Biomaterial Substrates (TTN-CBS) would more closely mimic natural biomass encountered in biorefining and new findings can be directly applied to improvement of raw material utilization.

KW - Bioethanol yield prediction

KW - Chromogenic substrates

KW - High-throughput enzyme screening

KW - Raw materials

UR - http://www.etaflorence.it/proceedings/

U2 - 10.5071/26thEUBCE2018-3CO.11.1

DO - 10.5071/26thEUBCE2018-3CO.11.1

M3 - Article in proceedings

AN - SCOPUS:85051019469

SN - 978-88-89407-18-9

T3 - European Biomass Conference and Exhibition Proceedings

SP - 979

EP - 983

BT - 26th European Biomass Conference and Exhibition Proceedings, 2018, Copenhagen

PB - ETA-Florence Renewable Energies

T2 - European Biomass Conference and Exhibition

Y2 - 14 May 2018 through 18 May 2018

ER -