Development of an analytical method for the metaproteomic investigation of bioaerosol from work environments

TY - JOUR

T1 - Development of an analytical method for the metaproteomic investigation of bioaerosol from work environments

AU - Piovesana, Susy

AU - Capriotti, Anna Laura

AU - Foglia, Patrizia

AU - Montone, Carmela Maria

AU - La Barbera, Giorgia

AU - Zenezini Chiozzi, Riccardo

AU - Laganà, Aldo

AU - Cavaliere, Chiara

N1 - (Ekstern)

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The metaproteomic analysis of air particulate matter provides valuable information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters is developed. Different protein extraction procedures are tested to select the best extraction protocol based on protein recovery. The optimized method is tested for the extraction of proteins from spores of ubiquitous bacteria species and used for the metaproteomic characterization of filters from three work environments. In particular, ambient aerosol samples are collected in a composting plant, in a wastewater treatment plant, and in an agricultural holding. A total of 179, 15, 205, and 444 proteins are identified in composting plant, wastewater treatment plant, and agricultural holding, (cow stable and blending plant), respectively. In agreement with the major categories of primary biological aerosol particles, all identified proteins originated primarily from fungi, bacteria, and plants. The paper is the first metaproteomic study applied to bioaerosol samples collected in occupationally relevant environmental sites and, even though not aimed at monitoring the risk exposure of workers, it provides information on the possible exposure in the working environmental sites.

AB - The metaproteomic analysis of air particulate matter provides valuable information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters is developed. Different protein extraction procedures are tested to select the best extraction protocol based on protein recovery. The optimized method is tested for the extraction of proteins from spores of ubiquitous bacteria species and used for the metaproteomic characterization of filters from three work environments. In particular, ambient aerosol samples are collected in a composting plant, in a wastewater treatment plant, and in an agricultural holding. A total of 179, 15, 205, and 444 proteins are identified in composting plant, wastewater treatment plant, and agricultural holding, (cow stable and blending plant), respectively. In agreement with the major categories of primary biological aerosol particles, all identified proteins originated primarily from fungi, bacteria, and plants. The paper is the first metaproteomic study applied to bioaerosol samples collected in occupationally relevant environmental sites and, even though not aimed at monitoring the risk exposure of workers, it provides information on the possible exposure in the working environmental sites.

KW - Agricultural holding

KW - Bioaerosols

KW - Composting plants

KW - Extraction methods

KW - High-resolution mass spectrometry

KW - Metaproteomics

KW - Wastewater treatment plants

UR - http://www.scopus.com/inward/record.url?scp=85074041205&partnerID=8YFLogxK

U2 - 10.1002/pmic.201900152

DO - 10.1002/pmic.201900152

M3 - Journal article

C2 - 31315163

AN - SCOPUS:85074041205

SN - 1615-9853

VL - 19

JO - Proteomics

JF - Proteomics

IS - 23

M1 - 1900152

ER -