TY - GEN
T1 - Development of a multiplex PCR assay detecting 52 autosomal SNPs
AU - Sanchez Sanchez, Juan Jose
AU - Phillips, C.
AU - Børsting, Claus
AU - Bogus, M.
AU - Carracedo, A.
AU - Syndercombe-Court, Denise
AU - Fondevila, M.
AU - Harrison, C.D.
AU - Morling, N.
AU - Balogh, K.
AU - Schneider, Peter M.
AU - SNPforID Consortium
PY - 2006
Y1 - 2006
N2 - An efficient method that can be used to simultaneously amplify a set of genetic loci across the genome with high reliability can provide a valuable tool for single nucleotide polymorphism (SNP) forensic genotyping. A crucial element is the number of individual biochemical reactions that must be performed. The SNPforID consortium (www.snpforid.org) was established in 2003 with the principal goal of developing a SNP-based system of DNA analysis that would have comparable discrimination power and ease of use to those of existing short tandem repeat (STR) based techniques. Here, we describe a strategy for amplifying 52 genomic DNA fragments, each containing one SNP, in a single tube, and accurately genotyping the PCR product mixture using two single base extension reactions. This multiplex approach reduces the cost of SNP genotyping and requires as little as 0.5 ng of genomic DNA to detect 52 SNPs. We used a multiple injection approach for DNA sequencers that can effectively detect all the SNPs amplified in a single electrophoretic run. We present SNP data for 700 unrelated individuals from 9 populations
AB - An efficient method that can be used to simultaneously amplify a set of genetic loci across the genome with high reliability can provide a valuable tool for single nucleotide polymorphism (SNP) forensic genotyping. A crucial element is the number of individual biochemical reactions that must be performed. The SNPforID consortium (www.snpforid.org) was established in 2003 with the principal goal of developing a SNP-based system of DNA analysis that would have comparable discrimination power and ease of use to those of existing short tandem repeat (STR) based techniques. Here, we describe a strategy for amplifying 52 genomic DNA fragments, each containing one SNP, in a single tube, and accurately genotyping the PCR product mixture using two single base extension reactions. This multiplex approach reduces the cost of SNP genotyping and requires as little as 0.5 ng of genomic DNA to detect 52 SNPs. We used a multiple injection approach for DNA sequencers that can effectively detect all the SNPs amplified in a single electrophoretic run. We present SNP data for 700 unrelated individuals from 9 populations
M3 - Article in proceedings
VL - 1288
T3 - ICS - International Congress Series
SP - 67
EP - 69
BT - Progress in Forensic Genetics 11
PB - Elsevier
T2 - 21st International ISFG Congress
Y2 - 13 September 2005 through 16 September 2005
ER -