Development and comparison of pH microoptodes for use in marine systems

Oliver Kohls*, Ingo Klimant, Gerhard A. Holst, Michael Kühl

*Corresponding author af dette arbejde
9 Citationer (Scopus)

Abstract

Traditionally microscale measurements of pH are based on potentiometric measurements with a pH glass microelectrode. The preparation of these electrodes is, however, very time consuming. We developed pH micro-optodes for use in seawater in the range of pH 7 - 9. The optodes are based on immobilized acid-base indicators, which change their color and/or fluorescence properties as a function of the pH. Various dyes were immobilized directly on the tip of a tapered optical fiber by different techniques. We then investigated these pH optodes with respect to response time, mechanical stability and calibration characteristics. Dependent on the optical properties of the indicator material we used different light emitting diodes (LED's) as the light sources and either a photodiode or a photomultiplier as detector.

OriginalsprogEngelsk
TidsskriftProceedings of SPIE - The International Society for Optical Engineering
Vol/bind2978
Sider (fra-til)82-91
Antal sider10
ISSN0277-786X
DOI
StatusUdgivet - 1 dec. 1997
BegivenhedMicro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications - San Jose, CA, USA
Varighed: 10 feb. 199710 feb. 1997

Konference

KonferenceMicro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications
Land/OmrådeUSA
BySan Jose, CA
Periode10/02/199710/02/1997

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