Development and application of an ELISA method for the analysis of protein-based binding media of artworks

Hae Young Lee; Natalya Atlasevich; Clara Granzotto; Julia Schultz; John Loike; Julie Arslanoglu

doi:10.1039/c4ay01919a

Development and application of an ELISA method for the analysis of protein-based binding media of artworks

Hae Young Lee, Natalya Atlasevich, Clara Granzotto, Julia Schultz, John Loike, Julie Arslanoglu

15 Citationer (Scopus)

Abstract

ELISA has been used extensively in scientific research and medical diagnostics since its invention in the 1960s. The application of ELISA to identify proteinaceous materials used in works from cultural heritage is a recent development and presents an important area of further investigation. This study introduces an optimized ELISA with a horse radish peroxidase (HRP) reporting system to identify select proteins in the adhesives and binders used in artworks. The described methodology is successfully applied to samples obtained from three different artworks from the Metropolitan Museum of Art. Furthermore, these experiments use protein quantitation to study the impact of specific pigments/binder combinations on the antigen detection by commercial antibodies. Our results suggest that the aging of specific pigment/binder combinations could impact the ELISA detection of proteins in artworks.

Originalsprog	Engelsk
Tidsskrift	Analytical Methods
Vol/bind	7
Udgave nummer	1
Sider (fra-til)	187-196
Antal sider	10
ISSN	1759-9660
DOI	https://doi.org/10.1039/c4ay01919a
Status	Udgivet - 7 jan. 2015

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T1 - Development and application of an ELISA method for the analysis of protein-based binding media of artworks

AU - Lee, Hae Young

AU - Atlasevich, Natalya

AU - Granzotto, Clara

AU - Schultz, Julia

AU - Loike, John

AU - Arslanoglu, Julie

PY - 2015/1/7

Y1 - 2015/1/7

N2 - ELISA has been used extensively in scientific research and medical diagnostics since its invention in the 1960s. The application of ELISA to identify proteinaceous materials used in works from cultural heritage is a recent development and presents an important area of further investigation. This study introduces an optimized ELISA with a horse radish peroxidase (HRP) reporting system to identify select proteins in the adhesives and binders used in artworks. The described methodology is successfully applied to samples obtained from three different artworks from the Metropolitan Museum of Art. Furthermore, these experiments use protein quantitation to study the impact of specific pigments/binder combinations on the antigen detection by commercial antibodies. Our results suggest that the aging of specific pigment/binder combinations could impact the ELISA detection of proteins in artworks.

AB - ELISA has been used extensively in scientific research and medical diagnostics since its invention in the 1960s. The application of ELISA to identify proteinaceous materials used in works from cultural heritage is a recent development and presents an important area of further investigation. This study introduces an optimized ELISA with a horse radish peroxidase (HRP) reporting system to identify select proteins in the adhesives and binders used in artworks. The described methodology is successfully applied to samples obtained from three different artworks from the Metropolitan Museum of Art. Furthermore, these experiments use protein quantitation to study the impact of specific pigments/binder combinations on the antigen detection by commercial antibodies. Our results suggest that the aging of specific pigment/binder combinations could impact the ELISA detection of proteins in artworks.

U2 - 10.1039/c4ay01919a

DO - 10.1039/c4ay01919a

M3 - Journal article

SN - 1759-9660

VL - 7

SP - 187

EP - 196

JO - Analytical Methods

JF - Analytical Methods

IS - 1

ER -

Development and application of an ELISA method for the analysis of protein-based binding media of artworks

Abstract

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