TY - JOUR
T1 - Determination of ptaquiloside and pterosin B derived from bracken (Pteridium aquilinum) in cattle plasma, urine and milk
AU - Aranha, Paulo Cesar Reis
AU - Hansen, Hans Chr. Bruun
AU - Rasmussen, Lars Holm
AU - Strobel, Bjarne W.
AU - Friis, Christian
PY - 2014/3/1
Y1 - 2014/3/1
N2 - Ptaquiloside (PTA) is a toxin from bracken fern (Pteridium sp.) with genotoxic effects. Hydrolysis of PTA leads to the non-toxic and aromatised indanone, pterosin B (PTB). Here we present a sensitive, fast, simple and direct method, using SPE cartridges to clean and pre-concentrate PTA and PTB in plasma, urine and milk followed by LC-MS quantification. The average recovery of PTA in plasma, urine, and milk was 71, 88 and 77%, respectively, whereas recovery of PTB was 75, 82 and 63%. The method LOQ for PTA and PTB in plasma was 1.2 and 3.7ngmL-1, 52 and 33ngmL-1 for undiluted urine and 5.8 and 5.3ngmL-1 for milk. The method is repeatable within and between days, with RSD values lower than 15% (PTA) and 20% (PTB). When PTA and PTB spiked samples were stored at -18°C for 14 days both compounds remained stable. In contrast, the PTA concentration was reduced by 15% when PTA spiked plasma was left for 5h at room temperature before SPE clean-up, whereas PTB remained stable. The method is the first to allow simultaneous quantification of PTA and PTB in biological fluids in a relevant concentration range. After intravenous administration of 0.092mg PTA per kgbw in a heifer, the plasma concentration was more than 300ngmL-1 PTA and declined to 9.8ngmL-1 after 6h, PTB was determined after 10min at 50ngmL-1.
AB - Ptaquiloside (PTA) is a toxin from bracken fern (Pteridium sp.) with genotoxic effects. Hydrolysis of PTA leads to the non-toxic and aromatised indanone, pterosin B (PTB). Here we present a sensitive, fast, simple and direct method, using SPE cartridges to clean and pre-concentrate PTA and PTB in plasma, urine and milk followed by LC-MS quantification. The average recovery of PTA in plasma, urine, and milk was 71, 88 and 77%, respectively, whereas recovery of PTB was 75, 82 and 63%. The method LOQ for PTA and PTB in plasma was 1.2 and 3.7ngmL-1, 52 and 33ngmL-1 for undiluted urine and 5.8 and 5.3ngmL-1 for milk. The method is repeatable within and between days, with RSD values lower than 15% (PTA) and 20% (PTB). When PTA and PTB spiked samples were stored at -18°C for 14 days both compounds remained stable. In contrast, the PTA concentration was reduced by 15% when PTA spiked plasma was left for 5h at room temperature before SPE clean-up, whereas PTB remained stable. The method is the first to allow simultaneous quantification of PTA and PTB in biological fluids in a relevant concentration range. After intravenous administration of 0.092mg PTA per kgbw in a heifer, the plasma concentration was more than 300ngmL-1 PTA and declined to 9.8ngmL-1 after 6h, PTB was determined after 10min at 50ngmL-1.
U2 - 10.1016/j.jchromb.2014.01.022
DO - 10.1016/j.jchromb.2014.01.022
M3 - Journal article
C2 - 24508676
SN - 1570-0232
VL - 951-952
SP - 44
EP - 51
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -