Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Christina Lazov; Mariann Chriél; Hans Baagøe; Esben Fjederholt; Yu Deng; Engbert Kooi; Graham Belsham; Anette Bøtner; Thomas Rasmussen

doi:10.3390/v10090486

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Christina Lazov, Mariann Chriél, Hans Baagøe, Esben Fjederholt, Yu Deng, Engbert Kooi, Graham Belsham, Anette Bøtner, Thomas Rasmussen

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Abstract

Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

Originalsprog	Engelsk
Artikelnummer	486
Tidsskrift	Viruses
Vol/bind	10
Udgave nummer	9
Sider (fra-til)	1-13
ISSN	1999-4915
DOI	https://doi.org/10.3390/v10090486
Status	Udgivet - 11 sep. 2018

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10.3390/v10090486Licens: CC BY

viruses-10-00486Licens: CC BY

Citationsformater

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abstract = "Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.",

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year = "2018",

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doi = "10.3390/v10090486",

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T1 - Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

AU - Lazov, Christina

AU - Chriél, Mariann

AU - Baagøe, Hans

AU - Fjederholt, Esben

AU - Deng, Yu

AU - Kooi, Engbert

AU - Belsham, Graham

AU - Bøtner, Anette

AU - Rasmussen, Thomas

PY - 2018/9/11

Y1 - 2018/9/11

N2 - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

AB - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

U2 - 10.3390/v10090486

DO - 10.3390/v10090486

M3 - Journal article

C2 - 30208582

SN - 1999-4915

VL - 10

SP - 1

EP - 13

JO - Viruses

JF - Viruses

IS - 9

M1 - 486

ER -

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Abstract

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