Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans

Danny Christiansen; Kasper Hvid Eibye; Villads Rasmussen; Hans Møller Voldby; Martin Thomassen; Michael Permin Nyberg; Thomas Gunnar Petursson Gunnarsson; Casper Skovgaard; Mads Søgaard Lindskrog; David J Bishop; Morten Hostrup; Jens Bangsbo

doi:10.1113/jp277657

Cycling with blood flow restriction improves performance and muscle K⁺ regulation and alters the effect of antioxidant infusion in humans

Danny Christiansen, Kasper Hvid Eibye, Villads Rasmussen, Hans Møller Voldby, Martin Thomassen, Michael Permin Nyberg, Thomas Gunnar Petursson Gunnarsson, Casper Skovgaard, Mads Søgaard Lindskrog, David J Bishop, Morten Hostrup, Jens Bangsbo

August Krogh Sektionen for Human Fysiologi

16 Citationer (Scopus)

Abstract

Key points: Training with blood flow restriction (BFR) is a well-recognized strategy for promoting muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. In the present study, we report that interval training with BFR augments improvements in performance and reduces net K⁺ release from contracting muscles during high-intensity exercise in active men. A better K⁺ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle anti-oxidant function, as indicated by a higher reduced to oxidized glutathione (GSH:GSSG) ratio, compared to control, as well as an increased thigh net K⁺ release during intense exercise with concomitant anti-oxidant infusion. Training with BFR also invoked fibre type-specific adaptations in the abundance of Na⁺,K⁺-ATPase isoforms (α₁, β₁, phospholemman/FXYD1). Thus, BFR-training enhances performance and K⁺ regulation during intense exercise, which may be a result of adaptations in anti-oxidant function, blood flow and Na⁺,K⁺-ATPase-isoform abundance at the fibre-type level. Abstract: We examined whether blood flow restriction (BFR) augments training-induced improvements in K⁺ regulation and performance during intense exercise in men, and also whether these adaptations are associated with an altered muscle anti-oxidant function, blood flow and/or with fibre type-dependent changes in Na⁺,K⁺-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 years, 49.7 ± 5.3 mL kg⁻¹ min⁻¹) performed 6 weeks of interval cycling, where one leg trained without BFR (control; CON-leg) and the other trained with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K⁺ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with i.v. infusion of N-acetylcysteine (NAC) or placebo (saline) and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, P < 0.05), whereas K⁺ release during Ex2 was attenuated only from BFR-leg (P < 0.05). The muscle GSH:GSSG ratio at rest and blood flow during exercise was higher in BFR-leg than in CON-leg after training (P < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K⁺ release during Ex2 only in BFR-leg (P < 0.05), whereas the abundance of Na⁺,K⁺-ATPase-isoform α₁ in type II (51%), β₁ in type I (33%), and FXYD1 in type I (108%) and type II (60%) fibres was higher in BFR-leg than in CON-leg (P < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K⁺ release during intense exercise, which were associated with adaptations in muscle anti-oxidant function, blood flow and Na⁺,K⁺-ATPase-isoform abundance at the fibre-type level.

Originalsprog	Engelsk
Tidsskrift	Journal of Physiology
Vol/bind	597
Udgave nummer	9
Sider (fra-til)	2421-2444
Antal sider	24
ISSN	0022-3751
DOI	https://doi.org/10.1113/jp277657
Status	Udgivet - 1 maj 2019

Emneord

Det Natur- og Biovidenskabelige Fakultet

Adgang til dokumentet

10.1113/jp277657

JP277657Forlagets udgivne version, 1,96 MB

Citationsformater

Christiansen, D., Eibye, K. H., Rasmussen, V., Voldby, H. M., Thomassen, M., Nyberg, M. P., Gunnarsson, T. G. P., Skovgaard, C., Lindskrog, M. S., Bishop, D. J., Hostrup, M., & Bangsbo, J. (2019). Cycling with blood flow restriction improves performance and muscle K⁺ regulation and alters the effect of antioxidant infusion in humans. Journal of Physiology, 597(9), 2421-2444. https://doi.org/10.1113/jp277657

Christiansen, D, Eibye, KH, Rasmussen, V, Voldby, HM, Thomassen, M, Nyberg, MP, Gunnarsson, TGP, Skovgaard, C, Lindskrog, MS, Bishop, DJ, Hostrup, M & Bangsbo, J 2019, 'Cycling with blood flow restriction improves performance and muscle K⁺ regulation and alters the effect of antioxidant infusion in humans', Journal of Physiology, bind 597, nr. 9, s. 2421-2444. https://doi.org/10.1113/jp277657

@article{54fde935e91a476f8da46243ba63de79,

title = "Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans",

abstract = "Key points: Training with blood flow restriction (BFR) is a well-recognized strategy for promoting muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. In the present study, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle anti-oxidant function, as indicated by a higher reduced to oxidized glutathione (GSH:GSSG) ratio, compared to control, as well as an increased thigh net K+ release during intense exercise with concomitant anti-oxidant infusion. Training with BFR also invoked fibre type-specific adaptations in the abundance of Na+,K+-ATPase isoforms (α1, β1, phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be a result of adaptations in anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level. Abstract: We examined whether blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and also whether these adaptations are associated with an altered muscle anti-oxidant function, blood flow and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 years, 49.7 ± 5.3 mL kg−1 min−1) performed 6 weeks of interval cycling, where one leg trained without BFR (control; CON-leg) and the other trained with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with i.v. infusion of N-acetylcysteine (NAC) or placebo (saline) and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, P < 0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (P < 0.05). The muscle GSH:GSSG ratio at rest and blood flow during exercise was higher in BFR-leg than in CON-leg after training (P < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (P < 0.05), whereas the abundance of Na+,K+-ATPase-isoform α1 in type II (51%), β1 in type I (33%), and FXYD1 in type I (108%) and type II (60%) fibres was higher in BFR-leg than in CON-leg (P < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level.",

keywords = "Faculty of Science, Blood flow restriction training, Human muscle, Antioxidant, Reactive oxygen species, N-acetylcysteine, Fibre type, Single type, Na+,K+-ATPase, Ion transport",

author = "Danny Christiansen and Eibye, {Kasper Hvid} and Villads Rasmussen and Voldby, {Hans M{\o}ller} and Martin Thomassen and Nyberg, {Michael Permin} and Gunnarsson, {Thomas Gunnar Petursson} and Casper Skovgaard and Lindskrog, {Mads S{\o}gaard} and Bishop, {David J} and Morten Hostrup and Jens Bangsbo",

year = "2019",

month = may,

day = "1",

doi = "10.1113/jp277657",

language = "English",

volume = "597",

pages = "2421--2444",

journal = "Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley-Blackwell",

number = "9",

}

TY - JOUR

T1 - Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of antioxidant infusion in humans

AU - Christiansen, Danny

AU - Eibye, Kasper Hvid

AU - Rasmussen, Villads

AU - Voldby, Hans Møller

AU - Thomassen, Martin

AU - Nyberg, Michael Permin

AU - Gunnarsson, Thomas Gunnar Petursson

AU - Skovgaard, Casper

AU - Lindskrog, Mads Søgaard

AU - Bishop, David J

AU - Hostrup, Morten

AU - Bangsbo, Jens

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Key points: Training with blood flow restriction (BFR) is a well-recognized strategy for promoting muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. In the present study, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle anti-oxidant function, as indicated by a higher reduced to oxidized glutathione (GSH:GSSG) ratio, compared to control, as well as an increased thigh net K+ release during intense exercise with concomitant anti-oxidant infusion. Training with BFR also invoked fibre type-specific adaptations in the abundance of Na+,K+-ATPase isoforms (α1, β1, phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be a result of adaptations in anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level. Abstract: We examined whether blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and also whether these adaptations are associated with an altered muscle anti-oxidant function, blood flow and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 years, 49.7 ± 5.3 mL kg−1 min−1) performed 6 weeks of interval cycling, where one leg trained without BFR (control; CON-leg) and the other trained with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with i.v. infusion of N-acetylcysteine (NAC) or placebo (saline) and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, P < 0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (P < 0.05). The muscle GSH:GSSG ratio at rest and blood flow during exercise was higher in BFR-leg than in CON-leg after training (P < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (P < 0.05), whereas the abundance of Na+,K+-ATPase-isoform α1 in type II (51%), β1 in type I (33%), and FXYD1 in type I (108%) and type II (60%) fibres was higher in BFR-leg than in CON-leg (P < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level.

AB - Key points: Training with blood flow restriction (BFR) is a well-recognized strategy for promoting muscle hypertrophy and strength. However, its potential to enhance muscle function during sustained, intense exercise remains largely unexplored. In the present study, we report that interval training with BFR augments improvements in performance and reduces net K+ release from contracting muscles during high-intensity exercise in active men. A better K+ regulation after BFR-training is associated with an elevated blood flow to exercising muscles and altered muscle anti-oxidant function, as indicated by a higher reduced to oxidized glutathione (GSH:GSSG) ratio, compared to control, as well as an increased thigh net K+ release during intense exercise with concomitant anti-oxidant infusion. Training with BFR also invoked fibre type-specific adaptations in the abundance of Na+,K+-ATPase isoforms (α1, β1, phospholemman/FXYD1). Thus, BFR-training enhances performance and K+ regulation during intense exercise, which may be a result of adaptations in anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level. Abstract: We examined whether blood flow restriction (BFR) augments training-induced improvements in K+ regulation and performance during intense exercise in men, and also whether these adaptations are associated with an altered muscle anti-oxidant function, blood flow and/or with fibre type-dependent changes in Na+,K+-ATPase-isoform abundance. Ten recreationally-active men (25 ± 4 years, 49.7 ± 5.3 mL kg−1 min−1) performed 6 weeks of interval cycling, where one leg trained without BFR (control; CON-leg) and the other trained with BFR (BFR-leg, pressure: ∼180 mmHg). Before and after training, femoral arterial and venous K+ concentrations and artery blood flow were measured during single-leg knee-extensor exercise at 25% (Ex1) and 90% of thigh incremental peak power (Ex2) with i.v. infusion of N-acetylcysteine (NAC) or placebo (saline) and a resting muscle biopsy was collected. After training, performance increased more in BFR-leg (23%) than in CON-leg (12%, P < 0.05), whereas K+ release during Ex2 was attenuated only from BFR-leg (P < 0.05). The muscle GSH:GSSG ratio at rest and blood flow during exercise was higher in BFR-leg than in CON-leg after training (P < 0.05). After training, NAC increased resting muscle GSH concentration and thigh net K+ release during Ex2 only in BFR-leg (P < 0.05), whereas the abundance of Na+,K+-ATPase-isoform α1 in type II (51%), β1 in type I (33%), and FXYD1 in type I (108%) and type II (60%) fibres was higher in BFR-leg than in CON-leg (P < 0.05). Thus, training with BFR elicited greater improvements in performance and reduced thigh K+ release during intense exercise, which were associated with adaptations in muscle anti-oxidant function, blood flow and Na+,K+-ATPase-isoform abundance at the fibre-type level.

KW - Faculty of Science

KW - Blood flow restriction training

KW - Human muscle

KW - Antioxidant

KW - Reactive oxygen species

KW - N-acetylcysteine

KW - Fibre type

KW - Single type

KW - Na+,K+-ATPase

KW - Ion transport

U2 - 10.1113/jp277657

DO - 10.1113/jp277657

M3 - Journal article

C2 - 30843602

SN - 0022-3751

VL - 597

SP - 2421

EP - 2444

JO - Journal of Physiology

JF - Journal of Physiology

IS - 9

ER -