Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes

TY - JOUR

T1 - Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes

AU - Roodbarkelari, Farshad

AU - Bramsiepe, Jonathan

AU - Weinl, Christina

AU - Marquardt, Sebastian

AU - Novák, Béla

AU - Jakoby, Marc J

AU - Lechner, Esther

AU - Genschik, Pascal

AU - Schnittger, Arp

PY - 2010/8/24

Y1 - 2010/8/24

N2 - One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles.

AB - One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Carrier Proteins

KW - Cell Cycle

KW - Cullin Proteins

KW - DNA Replication

KW - DNA, Plant

KW - Genes, Plant

KW - Microscopy, Electron, Scanning

KW - Models, Biological

KW - Mutation

KW - Plant Structures

KW - Plants, Genetically Modified

KW - Polyploidy

KW - RNA Interference

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1073/pnas.1006941107

DO - 10.1073/pnas.1006941107

M3 - Journal article

C2 - 20696906

SN - 0027-8424

VL - 107

SP - 15275

EP - 15280

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 34

ER -