Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module

San Hadzi; Abel Garcia-Pino; Kenn Gerdes; Jurij Lah; Remy Loris

doi:10.1107/s2053230x15000746

Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module

San Hadzi, Abel Garcia-Pino, Kenn Gerdes, Jurij Lah, Remy Loris

Functional genomics

1 Citationer (Scopus)

Abstract

The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221, with unit-cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P43212 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P41212, with unit-cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.

Originalsprog	Engelsk
Tidsskrift	Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online
Vol/bind	71
Udgave nummer	2
Sider (fra-til)	226-233
Antal sider	8
ISSN	1744-3091
DOI	https://doi.org/10.1107/s2053230x15000746
Status	Udgivet - 1 feb. 2015

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10.1107/s2053230x15000746

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Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module. / Hadzi, San; Garcia-Pino, Abel; Gerdes, Kenn et al.
I: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, Bind 71, Nr. 2, 01.02.2015, s. 226-233.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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AU - Loris, Remy

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AB - The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221, with unit-cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P43212 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P41212, with unit-cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.

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Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module

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