@inbook{f744a65d86364065ab30ec48b439964d,
title = "Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method",
abstract = "Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ({"}HiLiDe{"}) circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.",
author = "Oleg Sitsel and Kaituo Wang and Xiangyu Liu and Pontus Gourdon",
year = "2016",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-3179-8_37",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "1377",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "413--420",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
}