Crystallization of a stringent response factor from Aquifex aeolicus

Ole Kristensen; Martin Laurberg; Michael Gajhede

Crystallization of a stringent response factor from Aquifex aeolicus

Ole Kristensen, Martin Laurberg, Michael Gajhede

6 Citationer (Scopus)

Abstract

The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.

Originalsprog	Engelsk
Tidsskrift	Acta Crystallographica. Section D: Biological Crystallography
Vol/bind	58
Udgave nummer	Pt 7
Sider (fra-til)	1198-200
Antal sider	3
ISSN	0907-4449
Status	Udgivet - 2002

Citationsformater

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abstract = "The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.",

keywords = "Bacteria, Chromatography, Gel, Cloning, Molecular, Crystallography, X-Ray, Dimerization, Disulfides, Electrophoresis, Polyacrylamide Gel, Hydrogen Peroxide, Mutation, Phosphoprotein Phosphatases, Polymerase Chain Reaction, Software",

author = "Ole Kristensen and Martin Laurberg and Michael Gajhede",

year = "2002",

language = "English",

volume = "58",

pages = "1198--200",

journal = "Acta Crystallographica Section D: Structural Biology",

issn = "2059-7983",

publisher = "International Union of Crystallography",

number = "Pt 7",

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TY - JOUR

T1 - Crystallization of a stringent response factor from Aquifex aeolicus

AU - Kristensen, Ole

AU - Laurberg, Martin

AU - Gajhede, Michael

PY - 2002

Y1 - 2002

N2 - The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.

AB - The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.

KW - Bacteria

KW - Chromatography, Gel

KW - Cloning, Molecular

KW - Crystallography, X-Ray

KW - Dimerization

KW - Disulfides

KW - Electrophoresis, Polyacrylamide Gel

KW - Hydrogen Peroxide

KW - Mutation

KW - Phosphoprotein Phosphatases

KW - Polymerase Chain Reaction

KW - Software

M3 - Journal article

C2 - 12077442

SN - 2059-7983

VL - 58

SP - 1198

EP - 1200

JO - Acta Crystallographica Section D: Structural Biology

JF - Acta Crystallographica Section D: Structural Biology

IS - Pt 7

ER -

Crystallization of a stringent response factor from Aquifex aeolicus

Abstract

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