Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide

Paola Llinas, Marie Hélène Le Du, Henrik Gårdsvoll, Keld Danø, Michael Ploug, Bernard Gilquin, Enrico A Stura, Andre Ménèz

    193 Citationer (Scopus)

    Abstract

    We report the crystal structure of a soluble form of human urokinase-type plasminogen activator receptor (uPAR/CD87), which is expressed at the invasive areas of the tumor-stromal microenvironment in many human cancers. The structure was solved at 2.7 A in association with a competitive peptide inhibitor of the urokinase-type plasminogen activator (uPA)-uPAR interaction. uPAR is composed of three consecutive three-finger domains organized in an almost circular manner, which generates both a deep internal cavity where the peptide binds in a helical conformation, and a large external surface. This knowledge combined with the discovery of a convergent binding motif shared by the antagonist peptide and uPA allowed us to build a model of the human uPA-uPAR complex. This model reveals that the receptor-binding module of uPA engages the uPAR central cavity, thus leaving the external receptor surface accessible for other protein interactions (vitronectin and integrins). By this unique structural assembly, uPAR can orchestrate the fine interplay with the partners that are required to guide uPA-focalized proteolysis on the cell surface and control cell adhesion and migration.

    OriginalsprogEngelsk
    TidsskriftE M B O Journal
    Vol/bind24
    Udgave nummer9
    Sider (fra-til)1655-63
    Antal sider9
    ISSN0261-4189
    DOI
    StatusUdgivet - 4 maj 2005

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