TY - JOUR
T1 - Cooperation of B cells and T cells is required for survival of mice infected with vesicular stomatitis virus
AU - Thomsen, Allan Randrup
AU - Nansen, A
AU - Andersen, C
AU - Johansen, J
AU - Marker, O
AU - Christensen, Jan Pravsgaard
N1 - Keywords: Animals; Antigen-Presenting Cells; B-Lymphocytes; Central Nervous System Infections; Exons; Female; Immunoglobulin mu-Chains; Immunotherapy, Adoptive; Lymphocyte Cooperation; Male; Mice; Mice, Inbred Strains; Mice, Knockout; Mice, SCID; Rhabdoviridae Infections; Stomatitis; T-Lymphocyte Subsets; T-Lymphocytes; Vesicular stomatitis Indiana virus
PY - 1997
Y1 - 1997
N2 - To define the role of T cells and B cells in resistance to vesicular stomatitis virus (VSV) infection, knockout mice with different specific immune defects on an identical background were infected i.v. and the outcome of infection was compared; in this way a more complete picture of the relative importance of various host defence mechanisms could be obtained. Compared to T and B cell-deficient SCID mice which all succumbed from encephalitis within 5-9 days of infection, T cell-deficient nude mice generally lived longer, but within a period of approximately 1 month after challenge all died. In contrast, B cell-deficient mice were highly susceptible even to low doses of virus and mortality could be prevented by transfer of naive B cells prior to challenge as well as by immune serum given after challenge. Analysis of MHC class I- and class II-deficient mice revealed that CD8+ T cells could exert some antiviral activity, but CD4+ T cells sufficed for survival and were required for optimal resistance. Consistent with this it was found that in nude mice a lethal outcome could be prevented by transfer of CD8-depleted cells from B cell-deficient mice. Thus our results clearly demonstrate that while antibodies are pivotal for survival in the early phase of VSV infection, T cells are required for long-term survival, with CD4+ T cells being more effective in controlling this infection than CD8+ T cells.
AB - To define the role of T cells and B cells in resistance to vesicular stomatitis virus (VSV) infection, knockout mice with different specific immune defects on an identical background were infected i.v. and the outcome of infection was compared; in this way a more complete picture of the relative importance of various host defence mechanisms could be obtained. Compared to T and B cell-deficient SCID mice which all succumbed from encephalitis within 5-9 days of infection, T cell-deficient nude mice generally lived longer, but within a period of approximately 1 month after challenge all died. In contrast, B cell-deficient mice were highly susceptible even to low doses of virus and mortality could be prevented by transfer of naive B cells prior to challenge as well as by immune serum given after challenge. Analysis of MHC class I- and class II-deficient mice revealed that CD8+ T cells could exert some antiviral activity, but CD4+ T cells sufficed for survival and were required for optimal resistance. Consistent with this it was found that in nude mice a lethal outcome could be prevented by transfer of CD8-depleted cells from B cell-deficient mice. Thus our results clearly demonstrate that while antibodies are pivotal for survival in the early phase of VSV infection, T cells are required for long-term survival, with CD4+ T cells being more effective in controlling this infection than CD8+ T cells.
M3 - Journal article
C2 - 9418136
SN - 0953-8178
VL - 9
SP - 1757
EP - 1766
JO - International Immunology
JF - International Immunology
IS - 11
ER -