Continuous collection of volatiles produced by Streptomyces grown on oatmeal agar by headspace extraction and GC-MS

TY - GEN

T1 - Continuous collection of volatiles produced by Streptomyces grown on oatmeal agar by headspace extraction and GC-MS

AU - Podduturi, Raju

AU - Clausen, Nina

AU - Petersen, Nanna Bender

AU - Jørgensen, Niels O. G.

AU - Petersen, Mikael Agerlin

N1 - Conference code: 15

PY - 2018

Y1 - 2018

N2 - Volatile organic compounds (VOCs) produced by bacteria are known to play a significant role in interactions among many organisms, but VOCs also impart aroma to many food products. Streptomyces bacteria include a large group of organisms that produce a wide range of secondary volatile metabolites with potential for clinical and industrial applications. VOC profiles of bacteria are typically analyzed in liquid media. Yet, many bacteria also grow well on solid media and may here produce different VOCs than in liquid media. However, it is challenging to capture and measure VOCs from bacteria grown on solid media, and such limitations could bias measured VOCs profiles from bacteria. In this study, a special approach was applied to examine VOC production by Streptomyces when grown on oatmeal agar in a large gas-washing flask with Tenax-TA traps attached 96 hours of incubation. The obtained VOC profiles of two Streptomyces species show presence of geosmin and 2-MIB along with a total of 110 compounds, including 51 terpenes.

AB - Volatile organic compounds (VOCs) produced by bacteria are known to play a significant role in interactions among many organisms, but VOCs also impart aroma to many food products. Streptomyces bacteria include a large group of organisms that produce a wide range of secondary volatile metabolites with potential for clinical and industrial applications. VOC profiles of bacteria are typically analyzed in liquid media. Yet, many bacteria also grow well on solid media and may here produce different VOCs than in liquid media. However, it is challenging to capture and measure VOCs from bacteria grown on solid media, and such limitations could bias measured VOCs profiles from bacteria. In this study, a special approach was applied to examine VOC production by Streptomyces when grown on oatmeal agar in a large gas-washing flask with Tenax-TA traps attached 96 hours of incubation. The obtained VOC profiles of two Streptomyces species show presence of geosmin and 2-MIB along with a total of 110 compounds, including 51 terpenes.

U2 - 10.3217/978-3-85125-593-5

DO - 10.3217/978-3-85125-593-5

M3 - Article in proceedings

SN - 978-3-85125-593-5

SP - 475

EP - 478

BT - Flavour Science

A2 - Siegmund, Barbara

A2 - Leitner, Erich

PB - Verlag der Technische Universität Graz

T2 - Weurman Flavour Research Symposium 2017

Y2 - 18 September 2017 through 22 September 2017

ER -