TY - JOUR
T1 - Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene
AU - Bahl, Martin Iain
AU - Hansen, Lars Hestbjerg
AU - Sørensen, Søren Johannes
N1 - Keywords: Biosensor; Flow cytometry; Tetracycline
PY - 2005
Y1 - 2005
N2 - An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.
AB - An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml-1 to 16 µg ml-1, which represents a significant improvement of the original version.
U2 - 10.1016/j.femsle.2005.09.034
DO - 10.1016/j.femsle.2005.09.034
M3 - Journal article
C2 - 16239081
SN - 0378-1097
VL - 253
SP - 201
EP - 205
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -