Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes

TY - JOUR

T1 - Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes

AU - Andersen, Jens Bo

AU - Roldgaard, Bent

AU - Lindner, Ariel B

AU - Christensen, Bjarke B

AU - Licht, Tine R

PY - 2006

Y1 - 2006

N2 - Existing virulence models are often difficult to apply for quantitative comparison of invasion potentials of Listeria monocytogenes. Well-to-well variation between cell-line based in vitro assays is practically unavoidable, and variation between individual animals is the cause of large deviations in the observed capacity for infection when animal models are used. One way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. This, however, requires invasion-neutral markers that enable easy discrimination between the different strains.

AB - Existing virulence models are often difficult to apply for quantitative comparison of invasion potentials of Listeria monocytogenes. Well-to-well variation between cell-line based in vitro assays is practically unavoidable, and variation between individual animals is the cause of large deviations in the observed capacity for infection when animal models are used. One way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. This, however, requires invasion-neutral markers that enable easy discrimination between the different strains.

KW - Bacterial Proteins

KW - Caco-2 Cells

KW - Fluorescence

KW - Humans

KW - Listeria monocytogenes

KW - Listeriosis

KW - Staining and Labeling

KW - Virulence

U2 - 10.1186/1471-2180-6-86

DO - 10.1186/1471-2180-6-86

M3 - Journal article

C2 - 17014739

SN - 1471-2180

VL - 6

SP - 86

JO - B M C Microbiology

JF - B M C Microbiology

ER -