Comprehensive comparisons of the current human, mouse, and rat RefSeq, Ensembl, EST, and FANTOM3 datasets: identification of new human genes with specific tissue expression profile

TY - JOUR

T1 - Comprehensive comparisons of the current human, mouse, and rat RefSeq, Ensembl, EST, and FANTOM3 datasets

T2 - identification of new human genes with specific tissue expression profile

AU - Nordström, Karl J V

AU - Mirza, Majd A I

AU - Larsson, Thomas P

AU - Gloriam, David E.

AU - Fredriksson, Robert

AU - Schiöth, Helgi B

PY - 2006/9/29

Y1 - 2006/9/29

N2 - Our understanding of functional genetic elements in the genomes is continuously growing and new entries are entered in various databases on a regular basis. We have here merged the genetic elements in RefSeq, Ensembl, FANTOM3, HINV, and NCBI:s ESTdb using the genome assemblies in order to achieve a comprehensive picture of the current status of the identity and gene number in human, mouse, and rat. The number of human protein coding genes has not increased (25,043) while the increased sequencing of mouse transcripts has provided the considerably higher number of protein coding genes (31,578) in mouse. The results indicate large discrepancies between the datasets, as considerable numbers of unique transcripts can be found in each dataset. Despite the high number of ncRNA (38,129 in mouse) there are also almost 20,000 EST clusters in both mouse and humans with more than one EST that do not overlap any transcript suggesting that several new genetic elements are still to be found. We also demonstrated presence of new genes by identifying new human ones that have specific tissue profiles, using RT-PCR on rat tissues.

AB - Our understanding of functional genetic elements in the genomes is continuously growing and new entries are entered in various databases on a regular basis. We have here merged the genetic elements in RefSeq, Ensembl, FANTOM3, HINV, and NCBI:s ESTdb using the genome assemblies in order to achieve a comprehensive picture of the current status of the identity and gene number in human, mouse, and rat. The number of human protein coding genes has not increased (25,043) while the increased sequencing of mouse transcripts has provided the considerably higher number of protein coding genes (31,578) in mouse. The results indicate large discrepancies between the datasets, as considerable numbers of unique transcripts can be found in each dataset. Despite the high number of ncRNA (38,129 in mouse) there are also almost 20,000 EST clusters in both mouse and humans with more than one EST that do not overlap any transcript suggesting that several new genetic elements are still to be found. We also demonstrated presence of new genes by identifying new human ones that have specific tissue profiles, using RT-PCR on rat tissues.

KW - Animals

KW - Computational Biology

KW - Databases, Genetic

KW - Expressed Sequence Tags

KW - Gene Expression Profiling

KW - Genome

KW - Genome, Human

KW - Humans

KW - Mice

KW - Multigene Family

KW - Organ Specificity

KW - Rats

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Software

U2 - 10.1016/j.bbrc.2006.07.153

DO - 10.1016/j.bbrc.2006.07.153

M3 - Journal article

C2 - 16904064

SN - 0006-291X

VL - 348

SP - 1063

EP - 1074

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -