Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

Slawomir Jaworski; Ewa Sawosz; Marta Grodzik; Marta Kutwin; Mateusz Wierzbicki; Karolina Wlodyga; Agnieszka Jasik; Michał Reichert; André Chwalibog

doi:10.2478/bvip-2013-0101

Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

Slawomir Jaworski, Ewa Sawosz, Marta Grodzik, Marta Kutwin, Mateusz Wierzbicki, Karolina Wlodyga, Agnieszka Jasik, Michał Reichert, André Chwalibog

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Abstract

The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and U118 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.

Originalsprog	Engelsk
Tidsskrift	Bulletin of the Veterinary Institute in Pulawy
Vol/bind	57
Udgave nummer	4
Sider (fra-til)	593-598
Antal sider	6
ISSN	0042-4870
DOI	https://doi.org/10.2478/bvip-2013-0101
Status	Udgivet - dec. 2013

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10.2478/bvip-2013-0101

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Citationsformater

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title = "Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane",

abstract = "The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and U118 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.",

author = "Slawomir Jaworski and Ewa Sawosz and Marta Grodzik and Marta Kutwin and Mateusz Wierzbicki and Karolina Wlodyga and Agnieszka Jasik and Micha{\l} Reichert and Andr{\'e} Chwalibog",

year = "2013",

month = dec,

doi = "10.2478/bvip-2013-0101",

language = "English",

volume = "57",

pages = "593--598",

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TY - JOUR

T1 - Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

AU - Jaworski, Slawomir

AU - Sawosz, Ewa

AU - Grodzik, Marta

AU - Kutwin, Marta

AU - Wierzbicki, Mateusz

AU - Wlodyga, Karolina

AU - Jasik, Agnieszka

AU - Reichert, Michał

AU - Chwalibog, André

PY - 2013/12

Y1 - 2013/12

N2 - The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and U118 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.

AB - The objective of this study was the morphological and structural characterisation of glioblastoma multiforme grown in ovo. Glioma cells U87 and U118 were implanted in the chorioallantoic membrane (CAM) of chicken egg. After 10 days of incubation, tumours were resected for further analyses. Culturing two types of glioblastoma tumours from U87 and U118 cell lines has shown a number of differences in their morphology, histology, and ultrastructure. CAM assay proved to be a useful tool for studying glioblastoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of glioblastoma.

U2 - 10.2478/bvip-2013-0101

DO - 10.2478/bvip-2013-0101

M3 - Journal article

SN - 0042-4870

VL - 57

SP - 593

EP - 598

JO - Bulletin of the Veterinary Institute in Pulawy

JF - Bulletin of the Veterinary Institute in Pulawy

IS - 4

ER -

Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

Abstract

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