Comparison of semen quality between university-based and private assisted reproductive technology laboratories

Christian Fuglesang S Jensen; Omar Khan; Jens Sønksen; Mikkel Fode; James M Dupree; Tariq Shah; Dana A Ohl

doi:10.1080/21681805.2017.1409264

Comparison of semen quality between university-based and private assisted reproductive technology laboratories

Christian Fuglesang S Jensen, Omar Khan, Jens Sønksen, Mikkel Fode, James M Dupree, Tariq Shah, Dana A Ohl

Institut for Klinisk Medicin

1 Citationer (Scopus)

Abstract

OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen quality between private- and university-based assisted reproductive technology (ART) laboratories.

MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories. When more than one SA was available from either laboratory, the first at each laboratory was selected for analysis. Comparison of major semen parameters was performed using descriptive statistics and Bland-Altman plots, with differences tested using Wilcoxon-signed rank test.

RESULTS: Twenty-eight men aged 33 ± 5 (mean ± SD) years were included in the study. Motility was higher at the private laboratories compared to the university-based laboratory (Median difference -12.5%, 95% confidence interval -20.3%; -5.5%). Percent normal morphology was higher at the university-based laboratory compared to private laboratories (5.0%, 3.6%; 6.9%). No significant differences were found in volume, concentration and total motile sperm count although the Bland-Altman plot bias for concentration was clinically significant (15.9 × 106/ml).

CONCLUSIONS: In this small series, motility was significantly higher at private laboratories compared to a university-based laboratory but was above WHO reference limits at both places. Normal sperm morphology was significantly lower in semen analyses performed at private laboratories compared to a university-based laboratory and was below WHO reference limits.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Urology
Vol/bind	52
Udgave nummer	1
Sider (fra-til)	65-69
ISSN	0036-5599
DOI	https://doi.org/10.1080/21681805.2017.1409264
Status	Udgivet - 2 jan. 2018

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10.1080/21681805.2017.1409264

Citationsformater

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title = "Comparison of semen quality between university-based and private assisted reproductive technology laboratories",

abstract = "OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen quality between private- and university-based assisted reproductive technology (ART) laboratories.MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories. When more than one SA was available from either laboratory, the first at each laboratory was selected for analysis. Comparison of major semen parameters was performed using descriptive statistics and Bland-Altman plots, with differences tested using Wilcoxon-signed rank test.RESULTS: Twenty-eight men aged 33 ± 5 (mean ± SD) years were included in the study. Motility was higher at the private laboratories compared to the university-based laboratory (Median difference -12.5%, 95% confidence interval -20.3%; -5.5%). Percent normal morphology was higher at the university-based laboratory compared to private laboratories (5.0%, 3.6%; 6.9%). No significant differences were found in volume, concentration and total motile sperm count although the Bland-Altman plot bias for concentration was clinically significant (15.9 × 106/ml).CONCLUSIONS: In this small series, motility was significantly higher at private laboratories compared to a university-based laboratory but was above WHO reference limits at both places. Normal sperm morphology was significantly lower in semen analyses performed at private laboratories compared to a university-based laboratory and was below WHO reference limits.",

keywords = "Academic Medical Centers/standards, Adult, Humans, Laboratories/standards, Male, Private Practice/standards, Reproducibility of Results, Reproductive Techniques, Assisted/standards, Retrospective Studies, Semen, Semen Analysis/standards",

author = "Jensen, {Christian Fuglesang S} and Omar Khan and Jens S{\o}nksen and Mikkel Fode and Dupree, {James M} and Tariq Shah and Ohl, {Dana A}",

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doi = "10.1080/21681805.2017.1409264",

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T1 - Comparison of semen quality between university-based and private assisted reproductive technology laboratories

AU - Jensen, Christian Fuglesang S

AU - Khan, Omar

AU - Sønksen, Jens

AU - Fode, Mikkel

AU - Dupree, James M

AU - Shah, Tariq

AU - Ohl, Dana A

PY - 2018/1/2

Y1 - 2018/1/2

N2 - OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen quality between private- and university-based assisted reproductive technology (ART) laboratories.MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories. When more than one SA was available from either laboratory, the first at each laboratory was selected for analysis. Comparison of major semen parameters was performed using descriptive statistics and Bland-Altman plots, with differences tested using Wilcoxon-signed rank test.RESULTS: Twenty-eight men aged 33 ± 5 (mean ± SD) years were included in the study. Motility was higher at the private laboratories compared to the university-based laboratory (Median difference -12.5%, 95% confidence interval -20.3%; -5.5%). Percent normal morphology was higher at the university-based laboratory compared to private laboratories (5.0%, 3.6%; 6.9%). No significant differences were found in volume, concentration and total motile sperm count although the Bland-Altman plot bias for concentration was clinically significant (15.9 × 106/ml).CONCLUSIONS: In this small series, motility was significantly higher at private laboratories compared to a university-based laboratory but was above WHO reference limits at both places. Normal sperm morphology was significantly lower in semen analyses performed at private laboratories compared to a university-based laboratory and was below WHO reference limits.

AB - OBJECTIVE: Obtaining a semen analysis (SA) is an essential step in evaluating infertile men. Despite using standardized procedures for analysis semen quality in the same individual often varies on repeated tests. The objective of this study was to investigate inter-laboratory variation in semen quality between private- and university-based assisted reproductive technology (ART) laboratories.MATERIALS AND METHODS: IRB approval was obtained to retrospectively evaluate men with a SA at both the private- and university-based ART laboratories. When more than one SA was available from either laboratory, the first at each laboratory was selected for analysis. Comparison of major semen parameters was performed using descriptive statistics and Bland-Altman plots, with differences tested using Wilcoxon-signed rank test.RESULTS: Twenty-eight men aged 33 ± 5 (mean ± SD) years were included in the study. Motility was higher at the private laboratories compared to the university-based laboratory (Median difference -12.5%, 95% confidence interval -20.3%; -5.5%). Percent normal morphology was higher at the university-based laboratory compared to private laboratories (5.0%, 3.6%; 6.9%). No significant differences were found in volume, concentration and total motile sperm count although the Bland-Altman plot bias for concentration was clinically significant (15.9 × 106/ml).CONCLUSIONS: In this small series, motility was significantly higher at private laboratories compared to a university-based laboratory but was above WHO reference limits at both places. Normal sperm morphology was significantly lower in semen analyses performed at private laboratories compared to a university-based laboratory and was below WHO reference limits.

KW - Academic Medical Centers/standards

KW - Adult

KW - Humans

KW - Laboratories/standards

KW - Male

KW - Private Practice/standards

KW - Reproducibility of Results

KW - Reproductive Techniques, Assisted/standards

KW - Retrospective Studies

KW - Semen

KW - Semen Analysis/standards

U2 - 10.1080/21681805.2017.1409264

DO - 10.1080/21681805.2017.1409264

M3 - Journal article

C2 - 29191079

SN - 0036-5599

VL - 52

SP - 65

EP - 69

JO - Scandinavian Journal of Urology

JF - Scandinavian Journal of Urology

IS - 1

ER -

Comparison of semen quality between university-based and private assisted reproductive technology laboratories

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