Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents

Henrik Caspar Wegener; Dorte Lau Baggesen

Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents

Henrik Caspar Wegener, Dorte Lau Baggesen

3 Citationer (Scopus)

Abstract

Two commercial enzyme immunoassays, designated EIA-1 and EIA-2, for the detection of salmonella in feces and cecal contents were compared to conventional culture methods. Out of 362 cecal content samples, 35 were positive by EIA-1 and 30 were positive by EIA-2 and conventional methods. Out of 189 fecal samples, 41 were positive by EIA-1, 30 were positive by EIA-2, and 24 were positive by conventional culture methods. The EIA-1 assay method detected more positive samples overall, although only comparison of the fecal samples was statistically significant. The results indicate that the EIA-1 method is rapid, requiring only 24 hours, and may he more sensitive than the EIA-2 assay and conventional culture techniques. Enzyme immunoassays for the detection elf salmonella in fecal samples or cecal contents provide a reasonable alternative to conventional culture techniques.

Originalsprog	Engelsk
Tidsskrift	Journal of Veterinary Diagnostic Investigation
Vol/bind	9
Udgave nummer	4
Sider (fra-til)	352-356
ISSN	1040-6387
Status	Udgivet - 1997
Udgivet eksternt	Ja

Citationsformater

Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents. / Wegener, Henrik Caspar; Baggesen, Dorte Lau.

I: Journal of Veterinary Diagnostic Investigation, Bind 9, Nr. 4, 1997, s. 352-356.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents",

abstract = "Two commercial enzyme immunoassays, designated EIA-1 and EIA-2, for the detection of salmonella in feces and cecal contents were compared to conventional culture methods. Out of 362 cecal content samples, 35 were positive by EIA-1 and 30 were positive by EIA-2 and conventional methods. Out of 189 fecal samples, 41 were positive by EIA-1, 30 were positive by EIA-2, and 24 were positive by conventional culture methods. The EIA-1 assay method detected more positive samples overall, although only comparison of the fecal samples was statistically significant. The results indicate that the EIA-1 method is rapid, requiring only 24 hours, and may he more sensitive than the EIA-2 assay and conventional culture techniques. Enzyme immunoassays for the detection elf salmonella in fecal samples or cecal contents provide a reasonable alternative to conventional culture techniques.",

author = "Wegener, {Henrik Caspar} and Baggesen, {Dorte Lau}",

year = "1997",

language = "English",

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pages = "352--356",

journal = "Journal of Veterinary Diagnostic Investigation",

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TY - JOUR

T1 - Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents

AU - Wegener, Henrik Caspar

AU - Baggesen, Dorte Lau

PY - 1997

Y1 - 1997

N2 - Two commercial enzyme immunoassays, designated EIA-1 and EIA-2, for the detection of salmonella in feces and cecal contents were compared to conventional culture methods. Out of 362 cecal content samples, 35 were positive by EIA-1 and 30 were positive by EIA-2 and conventional methods. Out of 189 fecal samples, 41 were positive by EIA-1, 30 were positive by EIA-2, and 24 were positive by conventional culture methods. The EIA-1 assay method detected more positive samples overall, although only comparison of the fecal samples was statistically significant. The results indicate that the EIA-1 method is rapid, requiring only 24 hours, and may he more sensitive than the EIA-2 assay and conventional culture techniques. Enzyme immunoassays for the detection elf salmonella in fecal samples or cecal contents provide a reasonable alternative to conventional culture techniques.

AB - Two commercial enzyme immunoassays, designated EIA-1 and EIA-2, for the detection of salmonella in feces and cecal contents were compared to conventional culture methods. Out of 362 cecal content samples, 35 were positive by EIA-1 and 30 were positive by EIA-2 and conventional methods. Out of 189 fecal samples, 41 were positive by EIA-1, 30 were positive by EIA-2, and 24 were positive by conventional culture methods. The EIA-1 assay method detected more positive samples overall, although only comparison of the fecal samples was statistically significant. The results indicate that the EIA-1 method is rapid, requiring only 24 hours, and may he more sensitive than the EIA-2 assay and conventional culture techniques. Enzyme immunoassays for the detection elf salmonella in fecal samples or cecal contents provide a reasonable alternative to conventional culture techniques.

M3 - Journal article

SN - 1040-6387

VL - 9

SP - 352

EP - 356

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

IS - 4

ER -

Comparison of conventional culture methods and two commercial enzyme immunoassays for detection of Salmonella in porcine fecal samples and cecal contents

Abstract

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