COMPARISON OF CCD OF F, PARDE OF RP4, AND PARD OF R1 USING A NOVEL CONDITIONAL REPLICATION CONTROL-SYSTEM OF PLASMID R1

RB JENSEN; E GROHMANN; H SCHWAB; R DIAZOREJAS; Kenn Gerdes

doi:10.1111/j.1365-2958.1995.mmi17020211.x

COMPARISON OF CCD OF F, PARDE OF RP4, AND PARD OF R1 USING A NOVEL CONDITIONAL REPLICATION CONTROL-SYSTEM OF PLASMID R1

RB JENSEN, E GROHMANN, H SCHWAB, R DIAZOREJAS, Kenn Gerdes

71 Citationer (Scopus)

Abstract

A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchronous plasmid loss we constructed a novel plasmid replication-arrest system, which possesses the advantage that plasmid replication can be completely arrested by the addition of IPTG, a nonmetabolizable inducer, Using isogenic plasmid constructions we have found, for the first time, consistent correlation between the effect on steady-state loss rates and the effect on cell proliferation in the plasmid replication-arrest assay for all three systems. The parDE system had the most pronounced effect both on plasmid stabilization and on plasmid retention after replication arrest. In contrast, ccd and parD both exhibited weaker effects than anticipated from previously published results. Thus, our results indicate that the function and efficiencies of some of the systems should be reconsidered. Our results are consistent with the previously postulated hypothesis that ccd and parDE act by killing plasmid-free segregants, whereas parD seems to act by inhibiting cell division of plasmid-free segregants.

Originalsprog	Engelsk
Tidsskrift	Molecular Microbiology
Vol/bind	17
Udgave nummer	2
Sider (fra-til)	211-220
Antal sider	10
ISSN	0950-382X
DOI	https://doi.org/10.1111/j.1365-2958.1995.mmi17020211.x
Status	Udgivet - 1995
Udgivet eksternt	Ja

Adgang til dokumentet

10.1111/j.1365-2958.1995.mmi17020211.x

Citationsformater

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title = "COMPARISON OF CCD OF F, PARDE OF RP4, AND PARD OF R1 USING A NOVEL CONDITIONAL REPLICATION CONTROL-SYSTEM OF PLASMID R1",

abstract = "A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchronous plasmid loss we constructed a novel plasmid replication-arrest system, which possesses the advantage that plasmid replication can be completely arrested by the addition of IPTG, a nonmetabolizable inducer, Using isogenic plasmid constructions we have found, for the first time, consistent correlation between the effect on steady-state loss rates and the effect on cell proliferation in the plasmid replication-arrest assay for all three systems. The parDE system had the most pronounced effect both on plasmid stabilization and on plasmid retention after replication arrest. In contrast, ccd and parD both exhibited weaker effects than anticipated from previously published results. Thus, our results indicate that the function and efficiencies of some of the systems should be reconsidered. Our results are consistent with the previously postulated hypothesis that ccd and parDE act by killing plasmid-free segregants, whereas parD seems to act by inhibiting cell division of plasmid-free segregants.",

author = "RB JENSEN and E GROHMANN and H SCHWAB and R DIAZOREJAS and Kenn Gerdes",

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T1 - COMPARISON OF CCD OF F, PARDE OF RP4, AND PARD OF R1 USING A NOVEL CONDITIONAL REPLICATION CONTROL-SYSTEM OF PLASMID R1

AU - JENSEN, RB

AU - GROHMANN, E

AU - SCHWAB, H

AU - DIAZOREJAS, R

AU - Gerdes, Kenn

PY - 1995

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N2 - A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchronous plasmid loss we constructed a novel plasmid replication-arrest system, which possesses the advantage that plasmid replication can be completely arrested by the addition of IPTG, a nonmetabolizable inducer, Using isogenic plasmid constructions we have found, for the first time, consistent correlation between the effect on steady-state loss rates and the effect on cell proliferation in the plasmid replication-arrest assay for all three systems. The parDE system had the most pronounced effect both on plasmid stabilization and on plasmid retention after replication arrest. In contrast, ccd and parD both exhibited weaker effects than anticipated from previously published results. Thus, our results indicate that the function and efficiencies of some of the systems should be reconsidered. Our results are consistent with the previously postulated hypothesis that ccd and parDE act by killing plasmid-free segregants, whereas parD seems to act by inhibiting cell division of plasmid-free segregants.

AB - A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchronous plasmid loss we constructed a novel plasmid replication-arrest system, which possesses the advantage that plasmid replication can be completely arrested by the addition of IPTG, a nonmetabolizable inducer, Using isogenic plasmid constructions we have found, for the first time, consistent correlation between the effect on steady-state loss rates and the effect on cell proliferation in the plasmid replication-arrest assay for all three systems. The parDE system had the most pronounced effect both on plasmid stabilization and on plasmid retention after replication arrest. In contrast, ccd and parD both exhibited weaker effects than anticipated from previously published results. Thus, our results indicate that the function and efficiencies of some of the systems should be reconsidered. Our results are consistent with the previously postulated hypothesis that ccd and parDE act by killing plasmid-free segregants, whereas parD seems to act by inhibiting cell division of plasmid-free segregants.

U2 - 10.1111/j.1365-2958.1995.mmi17020211.x

DO - 10.1111/j.1365-2958.1995.mmi17020211.x

M3 - Journal article

SN - 0950-382X

VL - 17

SP - 211

EP - 220

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 2

ER -