Common polymorphisms in KCNJ5 [corrected] are associated with early-onset lone atrial fibrillation in Caucasians

Javad Jabbari; Morten S Olesen; Anders G Holst; Jonas B Nielsen; Stig Haunso; Jesper H Svendsen

doi:10.1159/000323840

Common polymorphisms in KCNJ5 [corrected] are associated with early-onset lone atrial fibrillation in Caucasians

Javad Jabbari, Morten S Olesen, Anders G Holst, Jonas B Nielsen, Stig Haunso, Jesper H Svendsen

Abstract

OBJECTIVES: The aim of this study was to screen lone atrial fibrillation (AF) patients for mutations in the genes KCNJ2, KCNJ3 and KCNJ5, all encoding potassium channels. Furthermore, we wanted to replicate the prior association of two single-nucleotide polymorphisms (SNPs) in KCNJ5, C171T (rs6590357) and G810T (rs7118824), with lone AF in Han Chinese.

METHODS: We sequenced the coding region and splice site of KCNJ2, KCNJ3 and KCNJ5 in 187 early-onset lone-AF patients screening for mutations and counting SNP frequencies for the two noted SNPs in KCNJ5.

RESULTS: No mutations were found in KCNJ2, KCNJ3 or KCNJ5. Both genotype distribution and allele frequencies of the SNPs rs6590357 and rs7118824 significantly differed between the AF and control group (p(genotype) = 0.0067, p(allele) = 0.0021 and p(genotype) = 0.014, p(allele) = 0.0101, respectively). On allele level, the OR for lone AF for rs6590357 was 1.77 (95% CI 1.16-2.73, p = 0.009) and for rs7118824 it was 1.71 (95% CI 1.13-2.57, p = 0.01) in a model adjusted for age and gender.

CONCLUSIONS: Our findings indicate that rs6590357 and rs7118824 in KCNJ5 are associated with early-onset lone AF in Caucasians. No mutations were found in the exon or splice site of KCNJ2, KCNJ3 or KCNJ5.

Originalsprog	Engelsk
Tidsskrift	Cardiology
Vol/bind	118
Udgave nummer	2
Sider (fra-til)	116-20
Antal sider	5
ISSN	0008-6312
DOI	https://doi.org/10.1159/000323840
Status	Udgivet - jun. 2011

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10.1159/000323840

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@article{4d0fc4567874453396c6ac9c70c23e70,

title = "Common polymorphisms in KCNJ5 [corrected] are associated with early-onset lone atrial fibrillation in Caucasians",

abstract = "OBJECTIVES: The aim of this study was to screen lone atrial fibrillation (AF) patients for mutations in the genes KCNJ2, KCNJ3 and KCNJ5, all encoding potassium channels. Furthermore, we wanted to replicate the prior association of two single-nucleotide polymorphisms (SNPs) in KCNJ5, C171T (rs6590357) and G810T (rs7118824), with lone AF in Han Chinese.METHODS: We sequenced the coding region and splice site of KCNJ2, KCNJ3 and KCNJ5 in 187 early-onset lone-AF patients screening for mutations and counting SNP frequencies for the two noted SNPs in KCNJ5.RESULTS: No mutations were found in KCNJ2, KCNJ3 or KCNJ5. Both genotype distribution and allele frequencies of the SNPs rs6590357 and rs7118824 significantly differed between the AF and control group (p(genotype) = 0.0067, p(allele) = 0.0021 and p(genotype) = 0.014, p(allele) = 0.0101, respectively). On allele level, the OR for lone AF for rs6590357 was 1.77 (95% CI 1.16-2.73, p = 0.009) and for rs7118824 it was 1.71 (95% CI 1.13-2.57, p = 0.01) in a model adjusted for age and gender.CONCLUSIONS: Our findings indicate that rs6590357 and rs7118824 in KCNJ5 are associated with early-onset lone AF in Caucasians. No mutations were found in the exon or splice site of KCNJ2, KCNJ3 or KCNJ5.",

keywords = "Adult, Aged, Atrial Fibrillation/genetics, Case-Control Studies, DNA Primers, Denmark, European Continental Ancestry Group, Female, G Protein-Coupled Inwardly-Rectifying Potassium Channels/genetics, Genotype, Humans, Logistic Models, Male, Middle Aged, Mutation, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Potassium Channels, Inwardly Rectifying/genetics, Young Adult",

author = "Javad Jabbari and Olesen, {Morten S} and Holst, {Anders G} and Nielsen, {Jonas B} and Stig Haunso and Svendsen, {Jesper H}",

note = "Copyright {\textcopyright} 2011 S. Karger AG, Basel.",

year = "2011",

month = jun,

doi = "10.1159/000323840",

language = "English",

volume = "118",

pages = "116--20",

journal = "Cardiology",

issn = "0008-6312",

publisher = "S Karger AG",

number = "2",

}

TY - JOUR

T1 - Common polymorphisms in KCNJ5 [corrected] are associated with early-onset lone atrial fibrillation in Caucasians

AU - Jabbari, Javad

AU - Olesen, Morten S

AU - Holst, Anders G

AU - Nielsen, Jonas B

AU - Haunso, Stig

AU - Svendsen, Jesper H

PY - 2011/6

Y1 - 2011/6

N2 - OBJECTIVES: The aim of this study was to screen lone atrial fibrillation (AF) patients for mutations in the genes KCNJ2, KCNJ3 and KCNJ5, all encoding potassium channels. Furthermore, we wanted to replicate the prior association of two single-nucleotide polymorphisms (SNPs) in KCNJ5, C171T (rs6590357) and G810T (rs7118824), with lone AF in Han Chinese.METHODS: We sequenced the coding region and splice site of KCNJ2, KCNJ3 and KCNJ5 in 187 early-onset lone-AF patients screening for mutations and counting SNP frequencies for the two noted SNPs in KCNJ5.RESULTS: No mutations were found in KCNJ2, KCNJ3 or KCNJ5. Both genotype distribution and allele frequencies of the SNPs rs6590357 and rs7118824 significantly differed between the AF and control group (p(genotype) = 0.0067, p(allele) = 0.0021 and p(genotype) = 0.014, p(allele) = 0.0101, respectively). On allele level, the OR for lone AF for rs6590357 was 1.77 (95% CI 1.16-2.73, p = 0.009) and for rs7118824 it was 1.71 (95% CI 1.13-2.57, p = 0.01) in a model adjusted for age and gender.CONCLUSIONS: Our findings indicate that rs6590357 and rs7118824 in KCNJ5 are associated with early-onset lone AF in Caucasians. No mutations were found in the exon or splice site of KCNJ2, KCNJ3 or KCNJ5.

AB - OBJECTIVES: The aim of this study was to screen lone atrial fibrillation (AF) patients for mutations in the genes KCNJ2, KCNJ3 and KCNJ5, all encoding potassium channels. Furthermore, we wanted to replicate the prior association of two single-nucleotide polymorphisms (SNPs) in KCNJ5, C171T (rs6590357) and G810T (rs7118824), with lone AF in Han Chinese.METHODS: We sequenced the coding region and splice site of KCNJ2, KCNJ3 and KCNJ5 in 187 early-onset lone-AF patients screening for mutations and counting SNP frequencies for the two noted SNPs in KCNJ5.RESULTS: No mutations were found in KCNJ2, KCNJ3 or KCNJ5. Both genotype distribution and allele frequencies of the SNPs rs6590357 and rs7118824 significantly differed between the AF and control group (p(genotype) = 0.0067, p(allele) = 0.0021 and p(genotype) = 0.014, p(allele) = 0.0101, respectively). On allele level, the OR for lone AF for rs6590357 was 1.77 (95% CI 1.16-2.73, p = 0.009) and for rs7118824 it was 1.71 (95% CI 1.13-2.57, p = 0.01) in a model adjusted for age and gender.CONCLUSIONS: Our findings indicate that rs6590357 and rs7118824 in KCNJ5 are associated with early-onset lone AF in Caucasians. No mutations were found in the exon or splice site of KCNJ2, KCNJ3 or KCNJ5.

KW - Adult

KW - Aged

KW - Atrial Fibrillation/genetics

KW - Case-Control Studies

KW - DNA Primers

KW - Denmark

KW - European Continental Ancestry Group

KW - Female

KW - G Protein-Coupled Inwardly-Rectifying Potassium Channels/genetics

KW - Genotype

KW - Humans

KW - Logistic Models

KW - Male

KW - Middle Aged

KW - Mutation

KW - Polymerase Chain Reaction

KW - Polymorphism, Single Nucleotide

KW - Potassium Channels, Inwardly Rectifying/genetics

KW - Young Adult

U2 - 10.1159/000323840

DO - 10.1159/000323840

M3 - Journal article

C2 - 21555883

SN - 0008-6312

VL - 118

SP - 116

EP - 120

JO - Cardiology

JF - Cardiology

IS - 2

ER -

Common polymorphisms in KCNJ5 [corrected] are associated with early-onset lone atrial fibrillation in Caucasians

Abstract

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