Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine

B. Gammelgaard; Claus Cornett; J. Olsen; L. Bendahl; S. H. Hansen

Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine

B. Gammelgaard, Claus Cornett, J. Olsen, L. Bendahl, S. H. Hansen

41 Citationer (Scopus)

Abstract

Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.

Originalsprog	Udefineret/Ukendt
Tidsskrift	Talanta
Vol/bind	59
Udgave nummer	6
Sider (fra-til)	1165-1171
Antal sider	7
ISSN	0039-9140
Status	Udgivet - 2003

Emneord

selenomethionine selenoxide selenium speciation mass-spectrometric detection selenium chromatography acid

Citationsformater

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T1 - Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine

AU - Gammelgaard, B.

AU - Cornett, Claus

AU - Olsen, J.

AU - Bendahl, L.

AU - Hansen, S. H.

PY - 2003

Y1 - 2003

N2 - Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.

AB - Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.

KW - selenomethionine selenoxide selenium speciation mass-spectrometric detection selenium chromatography acid

M3 - Tidsskriftartikel

SN - 0039-9140

VL - 59

SP - 1165

EP - 1171

JO - Talanta

JF - Talanta

IS - 6

ER -