Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men

Danny Christiansen; David John Bishop; James R Broatch; Jens Bangsbo; Michael John McKenna; Robyn M Murphy

doi:10.1152/japplphysiol.00259.2018

Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K⁺ transport proteins to sprint-interval training in men

Danny Christiansen, David John Bishop, James R Broatch, Jens Bangsbo, Michael John McKenna, Robyn M Murphy

August Krogh Sektionen for Human Fysiologi

10 Citationer (Scopus)

Abstract

Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally active men (24 6 yr, 79.5 10.8 kg, 44.6 5.8 ml·kg¹·min¹) completed six weeks of sprint-interval cycling, either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10°C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na, K-ATPase isoforms (1–3, 1–3) and phospholemman (FXYD1) and after recovery treatments (0 h and 3 h) on the first day of training to measure mRNA content. Training increased (P 0.05) the abundance of 1 and 3 in both fiber types and 1 in type-II fibers and decreased FXYD1 in type-I fibers, whereas 2 and 3 abundance was not altered by training (P 0.05). CWI after each session did not influence responses to training (P 0.05). However, 2 mRNA increased after the first session in COLD (0 h, P 0.05) but not in CON (P 0.05). In both conditions, 1 and 3 mRNA increased (3 h; P 0.05) and 2 mRNA decreased (3 h; P 0.05), whereas 3, 1, and FXYD1 mRNA remained unchanged (P 0.05) after the first session. In summary, Na,K-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which, for most isoforms, do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K regulation. cold-water immersion; fiber type; FXYD1; Na,K-ATPase; single-fiber western blotting; training.

Originalsprog	Engelsk
Tidsskrift	Journal of Applied Physiology
Vol/bind	125
Udgave nummer	2
Sider (fra-til)	429-444
Antal sider	16
ISSN	8750-7587
DOI	https://doi.org/10.1152/japplphysiol.00259.2018
Status	Udgivet - 2018

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Det Natur- og Biovidenskabelige Fakultet

Adgang til dokumentet

10.1152/japplphysiol.00259.2018

japplphysiol.00259.2018Forlagets udgivne version, 3,38 MB

Citationsformater

Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K⁺ transport proteins to sprint-interval training in men. / Christiansen, Danny; Bishop, David John; Broatch, James R et al.
I: Journal of Applied Physiology, Bind 125, Nr. 2, 2018, s. 429-444.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Cold-water immersion after training sessions: Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men",

abstract = "Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally active men (24 6 yr, 79.5 10.8 kg, 44.6 5.8 ml·kg1·min1) completed six weeks of sprint-interval cycling, either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10°C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na, K-ATPase isoforms (1–3, 1–3) and phospholemman (FXYD1) and after recovery treatments (0 h and 3 h) on the first day of training to measure mRNA content. Training increased (P 0.05) the abundance of 1 and 3 in both fiber types and 1 in type-II fibers and decreased FXYD1 in type-I fibers, whereas 2 and 3 abundance was not altered by training (P 0.05). CWI after each session did not influence responses to training (P 0.05). However, 2 mRNA increased after the first session in COLD (0 h, P 0.05) but not in CON (P 0.05). In both conditions, 1 and 3 mRNA increased (3 h; P 0.05) and 2 mRNA decreased (3 h; P 0.05), whereas 3, 1, and FXYD1 mRNA remained unchanged (P 0.05) after the first session. In summary, Na,K-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which, for most isoforms, do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K regulation. cold-water immersion; fiber type; FXYD1; Na,K-ATPase; single-fiber western blotting; training.",

keywords = "Faculty of Science, Cold water immersion, Training, Human muscle, Fibre type, Ion transport, FXYD1, Na+,K+-ATPAse",

author = "Danny Christiansen and Bishop, {David John} and Broatch, {James R} and Jens Bangsbo and McKenna, {Michael John} and Murphy, {Robyn M}",

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T1 - Cold-water immersion after training sessions

T2 - Effects on fiber type-specific adaptations in muscle K+ transport proteins to sprint-interval training in men

AU - Christiansen, Danny

AU - Bishop, David John

AU - Broatch, James R

AU - Bangsbo, Jens

AU - McKenna, Michael John

AU - Murphy, Robyn M

N1 - CURIS 2018 NEXS 298

PY - 2018

Y1 - 2018

N2 - Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally active men (24 6 yr, 79.5 10.8 kg, 44.6 5.8 ml·kg1·min1) completed six weeks of sprint-interval cycling, either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10°C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na, K-ATPase isoforms (1–3, 1–3) and phospholemman (FXYD1) and after recovery treatments (0 h and 3 h) on the first day of training to measure mRNA content. Training increased (P 0.05) the abundance of 1 and 3 in both fiber types and 1 in type-II fibers and decreased FXYD1 in type-I fibers, whereas 2 and 3 abundance was not altered by training (P 0.05). CWI after each session did not influence responses to training (P 0.05). However, 2 mRNA increased after the first session in COLD (0 h, P 0.05) but not in CON (P 0.05). In both conditions, 1 and 3 mRNA increased (3 h; P 0.05) and 2 mRNA decreased (3 h; P 0.05), whereas 3, 1, and FXYD1 mRNA remained unchanged (P 0.05) after the first session. In summary, Na,K-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which, for most isoforms, do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K regulation. cold-water immersion; fiber type; FXYD1; Na,K-ATPase; single-fiber western blotting; training.

AB - Effects of regular use of cold-water immersion (CWI) on fiber type-specific adaptations in muscle K transport proteins to intense training, along with their relationship to changes in mRNA levels after the first training session, were investigated in humans. Nineteen recreationally active men (24 6 yr, 79.5 10.8 kg, 44.6 5.8 ml·kg1·min1) completed six weeks of sprint-interval cycling, either without (passive rest; CON) or with training sessions followed by CWI (15 min at 10°C; COLD). Muscle biopsies were obtained before and after training to determine abundance of Na, K-ATPase isoforms (1–3, 1–3) and phospholemman (FXYD1) and after recovery treatments (0 h and 3 h) on the first day of training to measure mRNA content. Training increased (P 0.05) the abundance of 1 and 3 in both fiber types and 1 in type-II fibers and decreased FXYD1 in type-I fibers, whereas 2 and 3 abundance was not altered by training (P 0.05). CWI after each session did not influence responses to training (P 0.05). However, 2 mRNA increased after the first session in COLD (0 h, P 0.05) but not in CON (P 0.05). In both conditions, 1 and 3 mRNA increased (3 h; P 0.05) and 2 mRNA decreased (3 h; P 0.05), whereas 3, 1, and FXYD1 mRNA remained unchanged (P 0.05) after the first session. In summary, Na,K-ATPase isoforms are differently regulated in type I and II muscle fibers by sprint-interval training in humans, which, for most isoforms, do not associate with changes in mRNA levels after the first training session. CWI neither impairs nor improves protein adaptations to intense training of importance for muscle K regulation. cold-water immersion; fiber type; FXYD1; Na,K-ATPase; single-fiber western blotting; training.

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KW - Human muscle

KW - Fibre type

KW - Ion transport

KW - FXYD1

KW - Na+,K+-ATPAse

U2 - 10.1152/japplphysiol.00259.2018

DO - 10.1152/japplphysiol.00259.2018

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